Khan M M, Strober S, Melmon K L
Cell Immunol. 1986 Nov;103(1):41-53. doi: 10.1016/0008-8749(86)90066-3.
We have investigated the role of mast cells as modulators of lymphocyte function because the mast cells are concentrated in the areas of lymphoid storage; they are dependent upon T-cell growth factor for their proliferation; and they appear to be the principle if not sole storage site for histamine. We have tested the influence of mast cells on the proliferation of alloreactive cloned helper T cells, mixed leukocyte reactions, and the suppressive capacity of natural suppressor cells. We used an IL-3-dependent mast cell line that at high numbers (greater than 10(5)) suppressed and at low numbers (10(3) to 6 X 10(4)) augmented the proliferation of TH cells. Addition of histamine to cocultures enhanced the mast cell mediated proliferation of TH cells without directly affecting the helper cells. The action of histamine appeared to be mediated with H1 type receptors on these mast cells. Pretreatment of natural suppressor cells with supernatants from mast cell enhanced their suppressive capability. Here too, histamines enhanced suppression by the NS cell via histamine type 1 receptors on the natural suppressor cells. Our data suggest that mast cells may be a major modulator of the lymphoid cell immune function and demonstrate a role of histamine type 1 receptors in the interaction between mast cells, helper T cells, and natural suppressor cells.
我们研究了肥大细胞作为淋巴细胞功能调节因子的作用，因为肥大细胞集中在淋巴储存区域；它们的增殖依赖于T细胞生长因子；而且它们似乎是组胺的主要（如果不是唯一的）储存部位。我们测试了肥大细胞对同种异体反应性克隆辅助性T细胞增殖、混合淋巴细胞反应以及天然抑制细胞抑制能力的影响。我们使用了一种依赖白细胞介素-3的肥大细胞系，该细胞系在数量较多时（大于10⁵）会抑制TH细胞的增殖，而在数量较少时（10³至6×10⁴）则会增强TH细胞的增殖。向共培养物中添加组胺可增强肥大细胞介导的TH细胞增殖，而不会直接影响辅助性细胞。组胺的作用似乎是通过这些肥大细胞上的H1型受体介导的。用肥大细胞的上清液预处理天然抑制细胞可增强其抑制能力。同样，组胺通过天然抑制细胞上的组胺1型受体增强了NS细胞的抑制作用。我们的数据表明，肥大细胞可能是淋巴细胞免疫功能的主要调节因子，并证明了组胺1型受体在肥大细胞、辅助性T细胞和天然抑制细胞之间相互作用中的作用。
