Djuricic B M, Ueki Y, Spatz M
J Neurochem. 1985 Jun;44(6):1920-4. doi: 10.1111/j.1471-4159.1985.tb07188.x.
A combined method is described for the determination of various metabolites from a single tissue sample of the brain. It comprises a quick inactivation of cerebral enzymes by microwave irradiation, easy separation of the desired brain regions, and perchloric acid extraction of tissue substances, which are assayed either by specific enzymatic techniques or by HPLC with electrochemical detection. The obtained values of most energy and neurotransmitter metabolites in the brain are in agreement with those reported using other methods. However, this technique, in contrast to the brain freezing in vitro or freeze-blowing, provides a more efficient procedure for rapid arrest of cerebral metabolism even in the deep brain structures and is therefore suitable for detection of early changes particularly those occurring in experimental pathological conditions such as ischemia.
本文描述了一种从大脑单一组织样本中测定多种代谢物的联合方法。该方法包括通过微波辐射快速灭活脑酶、轻松分离所需的脑区，以及用高氯酸提取组织物质，这些物质可通过特定的酶技术或电化学检测的高效液相色谱法进行测定。大脑中大多数能量和神经递质代谢物的测定值与使用其他方法报告的值一致。然而，与体外脑冷冻或冷冻吹干相比，该技术为快速停止大脑代谢提供了更有效的程序，即使在深部脑结构中也是如此，因此适用于检测早期变化，特别是在实验性病理条件（如缺血）下发生的变化。
