Morel G, Leroux P, Pelletier G
Neuropeptides. 1985 Mar;6(1):41-52. doi: 10.1016/0143-4179(85)90129-5.
The localization and characterization of somatostatin-14 (SS-14) and somatostatin-28 (SS-28) receptors were studied in the rat pituitary using the iodinated agonists [Tyr0, D-Trp8]-SS-14 and [Leu8, D-Trp22, Tyr25]-SS-28 as tracers. Slide-mounted frozen sections were used for the autoradiographic localization and biochemical characterization of somatostatin receptors. In the latter case, counting was performed on scraped off serial sections which contained both anterior and posterior pituitary. Specificity studies demonstrated that either tracer could be displaced with SS-28 and SS-14. A number of unrelated peptides were unable to compete with either tracer. Scatchard analysis of saturation curves gave one-site interactions with KD values of 0.53 +/- 0.08 nM and 0.22 +/- 0.03 nM for the SS-14 and SS-28 iodinated agonists, respectively. Using autoradiography, SS-14 and SS-28 receptors were shown to be distributed in the anterior pituitary only. Using densitometry, displacement and saturation curves could be also obtained.
利用碘化激动剂[酪氨酸⁰，D-色氨酸⁸]-生长抑素-14（SS-14）和[亮氨酸⁸，D-色氨酸²²，酪氨酸²⁵]-生长抑素-28（SS-28）作为示踪剂，研究了生长抑素-14（SS-14）和生长抑素-28（SS-28）受体在大鼠垂体中的定位和特性。载玻片上的冰冻切片用于生长抑素受体的放射自显影定位和生化特性分析。在后一种情况下，对刮下的包含垂体前叶和后叶的连续切片进行计数。特异性研究表明，两种示踪剂均可被SS-28和SS-14取代。许多不相关的肽不能与任何一种示踪剂竞争。对饱和曲线进行Scatchard分析，结果显示与SS-14和SS-28碘化激动剂的单点相互作用的解离常数（KD）值分别为0.53±0.08 nM和0.22±0.03 nM。通过放射自显影显示，SS-14和SS-28受体仅分布在垂体前叶。使用密度测定法，也可获得置换曲线和饱和曲线。
