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Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1.
Mol Cell. 2017 Jul 6;67(1):139-147.e2. doi: 10.1016/j.molcel.2017.04.019. Epub 2017 Jun 6.
2
Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.
Mol Cell. 2017 Aug 17;67(4):633-645.e3. doi: 10.1016/j.molcel.2017.06.035. Epub 2017 Aug 3.
3
Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Aug;35(8):789-792. doi: 10.1038/nbt.3900. Epub 2017 Jun 5.
4
The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs.
FEMS Microbiol Lett. 2019 Apr 1;366(8). doi: 10.1093/femsle/fnz085.
5
Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
Mol Cell. 2017 Apr 20;66(2):221-233.e4. doi: 10.1016/j.molcel.2017.03.016.
6
Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a).
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5444-5449. doi: 10.1073/pnas.1718686115. Epub 2018 May 7.
7
Structural Variation of Type I-F CRISPR RNA Guided DNA Surveillance.
Mol Cell. 2017 Aug 17;67(4):622-632.e4. doi: 10.1016/j.molcel.2017.06.036. Epub 2017 Aug 3.
8
C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism.
Mol Cell. 2017 Jan 19;65(2):310-322. doi: 10.1016/j.molcel.2016.11.040. Epub 2016 Dec 15.
9
Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a.
Mol Cell. 2018 Sep 6;71(5):816-824.e3. doi: 10.1016/j.molcel.2018.06.043. Epub 2018 Aug 2.
10
Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9.
Mol Cell. 2017 Jul 6;67(1):117-127.e5. doi: 10.1016/j.molcel.2017.05.024. Epub 2017 Jun 9.

引用本文的文献

1
Conformational changes induced by K949A mutation in the CRISPR-Cas12a complex drives an effective target-binding mechanism.
Curr Res Struct Biol. 2025 Aug 8;10:100173. doi: 10.1016/j.crstbi.2025.100173. eCollection 2025 Dec.
2
CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction.
Fundam Res. 2022 Jul 15;5(4):1481-1487. doi: 10.1016/j.fmre.2022.06.017. eCollection 2025 Jul.
3
ENHANCED CLEAVAGE OF GENOMIC USING CASX2.
bioRxiv. 2025 Jul 11:2025.07.08.663680. doi: 10.1101/2025.07.08.663680.
4
Directed evolution expands CRISPR-Cas12a genome-editing capacity.
Nucleic Acids Res. 2025 Jul 8;53(13). doi: 10.1093/nar/gkaf649.
8
AlPaCas: allele-specific CRISPR gene editing through a protospacer-adjacent-motif (PAM) approach.
Nucleic Acids Res. 2024 Jul 5;52(W1):W29-W38. doi: 10.1093/nar/gkae419.
9
Discovery and structural mechanism of DNA endonucleases guided by RAGATH-18-derived RNAs.
Cell Res. 2024 May;34(5):370-385. doi: 10.1038/s41422-024-00952-1. Epub 2024 Apr 4.
10
Utilization of nicking properties of CRISPR-Cas12a effector for genome editing.
Sci Rep. 2024 Feb 9;14(1):3352. doi: 10.1038/s41598-024-53648-2.

本文引用的文献

1
Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Aug;35(8):789-792. doi: 10.1038/nbt.3900. Epub 2017 Jun 5.
2
Diversity and evolution of class 2 CRISPR-Cas systems.
Nat Rev Microbiol. 2017 Mar;15(3):169-182. doi: 10.1038/nrmicro.2016.184. Epub 2017 Jan 23.
3
In vivo high-throughput profiling of CRISPR-Cpf1 activity.
Nat Methods. 2017 Feb;14(2):153-159. doi: 10.1038/nmeth.4104. Epub 2016 Dec 19.
4
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
5
Structures and mechanisms of CRISPR RNA-guided effector nucleases.
Curr Opin Struct Biol. 2017 Apr;43:68-78. doi: 10.1016/j.sbi.2016.11.013. Epub 2016 Nov 30.
6
Applications of CRISPR technologies in research and beyond.
Nat Biotechnol. 2016;34(9):933-941. doi: 10.1038/nbt.3659. Epub 2016 Sep 8.
7
Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems.
Science. 2016 Aug 5;353(6299):aad5147. doi: 10.1126/science.aad5147.
8
Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition.
Cell Res. 2016 Aug;26(8):901-13. doi: 10.1038/cr.2016.88. Epub 2016 Jul 22.
9
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.
Nat Biotechnol. 2016 Aug;34(8):869-74. doi: 10.1038/nbt.3620. Epub 2016 Jun 27.
10
Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells.
Nat Biotechnol. 2016 Aug;34(8):863-8. doi: 10.1038/nbt.3609. Epub 2016 Jun 6.

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