Joo Jae-Hyoung, Kuang Zhen, Wang Pengbin, Park Bum Soo, Patidar Shailesh Kumar, Han Myung-Soo
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
Department of Environment Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
Environ Pollut. 2017 Oct;229:735-745. doi: 10.1016/j.envpol.2017.02.045. Epub 2017 Jun 7.
The novel eco-friendly algaecidal naphthoquinone derivate was used to control harmful algal bloom causing species Stephanodiscus and, its effect was assessed on other undesired and non-targeted microbial communities. We conducted a mesocosm experiment to investigate the effects of this novel algaecide on native microbial communities rearing in water collected from Nakdonggang River. Upon treatment of the mesocosm with the naphthoquinone derivate the concentration of Chl-a decreased from 20.4 μg L to 9.5 μg L after 2 days. The turbidity has also shown decrement (exhibited 15.5 NTU on the 7th day). The concentrations of DOC and phosphate in the treatment were slightly higher than those in the control due to the decomposition of dead Stephanodiscus, whereas the DO and pH in the treated condition were slightly lower than those in the control; which was due to increment of organic acids and higher degradation activity. Results showed that bacterial abundance were not significantly different but community composition were slightly different as revealed by NGS (Next generation sequencing). The variation in HNF (Heterotrophic nanoflagellates) revealed that the bacterial community composition changed following the change in bacterial abundance. During the treatment, the abundance of Stephanodiscus was significantly reduced by more than 80% after 6 days, and the abundance of ciliates and the dominant species, Halteria grandinella, had shown marked decline. The abundance of zooplankton sharply decreased to 5 ind. Lon the 8th day but increased again by the end of the study period. The Shannon-Wiener diversity index of phytoplankton, ciliates and zooplankton in the treated mesocosm increased significantly after 4, 7 and 8 days, respectively. The marked changes in the ecosystem structure were observed in treatment compare to control. However, the beneficial microalgal populations were not affected which indicated possibility of restoration of treated ecosystem and regain of healthy community structure after certain period.
这种新型环保型杀藻萘醌衍生物被用于控制导致有害藻华的物种小环藻，并且评估了其对其他非期望的和非靶向微生物群落的影响。我们进行了一项中宇宙实验，以研究这种新型杀藻剂对从洛东江采集的水中原生微生物群落的影响。在用萘醌衍生物处理中宇宙后，叶绿素a的浓度在2天后从20.4微克/升降至9.5微克/升。浊度也有所下降（第7天为15.5 NTU）。由于死亡的小环藻分解，处理组中的溶解有机碳和磷酸盐浓度略高于对照组，而处理条件下的溶解氧和pH值略低于对照组；这是由于有机酸增加和更高的降解活性所致。结果表明，细菌丰度没有显著差异，但通过NGS（下一代测序）揭示群落组成略有不同。异养纳米鞭毛虫的变化表明细菌群落组成随细菌丰度的变化而改变。在处理过程中，小环藻的丰度在6天后显著降低了80%以上，纤毛虫和优势物种大型弹跳虫的丰度显著下降。浮游动物的丰度在第8天急剧下降至5个/升，但在研究期结束时又有所增加。处理后的中宇宙中浮游植物、纤毛虫和浮游动物的香农-威纳多样性指数分别在第4、7和8天后显著增加。与对照组相比，处理组的生态系统结构发生了显著变化。然而，有益微藻种群未受影响，这表明经过一定时期后，处理后的生态系统有可能恢复并重新获得健康的群落结构。
