Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Bioresour Technol. 2017 Dec;245(Pt B):1455-1460. doi: 10.1016/j.biortech.2017.05.127. Epub 2017 May 22.
The aim of this work was to study the feasibility of surface displaying synthetic phytochelatin (EC) on Saccharomyces cerevisiae to overcome the inhibitory effect of heavy metals on ethanol production. Via the fusion of a gene encoding EC to an α-agglutinin gene, the engineered S. cerevisiae was able to successfully display EC on its surface. This surface engineered yeast strain exhibited an efficient cadmium adsorption capability and a remarkably enhanced cadmium tolerance. Moreover, its ethanol production efficiency was significantly improved as compared to a control strain in the presence of cadmium. Similar results could also be observed in the presence of other metals, such as nickel, lead and copper. Overall, this method allows simultaneous biorefinery and heavy metal removal when using heavy metal-contaminated biomass as raw materials.
本研究旨在探讨在酿酒酵母表面展示合成植物螯合肽（EC）以克服重金属对乙醇生产抑制作用的可行性。通过将编码 EC 的基因与 α-凝集素基因融合，成功实现了 EC 在酵母表面的展示。这种表面工程化酵母菌株表现出高效的镉吸附能力和显著增强的镉耐受性。此外，与对照菌株相比，在存在镉的情况下，其乙醇生产效率显著提高。在存在其他金属如镍、铅和铜的情况下，也观察到了类似的结果。总的来说，当使用重金属污染的生物质作为原料时，该方法允许同时进行生物炼制和重金属去除。
