Hassanpour Parisa, Amirfarhangi Abdollah, Hosseini-Fard Syed Reza, Yarnazari Amaneh, Najafi Mohammad
Iran University of Medical Sciences, School of Medicine, International Branch, Tehran, Iran.
Shahid Rajaee Hospital, Iran University of Medical Sciences, Tehran, Iran.
Gene. 2017 Aug 30;626:442-446. doi: 10.1016/j.gene.2017.05.063. Epub 2017 Jun 6.
Macrophages are known as important immune cells involved in the improvement of atherosclerosis plaques. The M2 macrophages are beneficial because scavenging the non-functional components in vessel sub-endothelial space. In this study, we investigated the effects of small dense LDL (sdLDL) on the changes of indoleamine 2,3-dioxygense (IDO) and interleukin (IL6) in the differentiated M2 macrophages. The patients were selected from who underwent coronary artery angiography. The monocytes were isolated from the whole blood samples of healthy (<5% stenosis) and patient (>70% stenosis; SVD, 2VD and 3VD) subjects and, were differentiated into M2 macrophages. The IDO gene expression, activity and IL6 values were measured by RT-qPCR, colorimetry and ELISA techniques, respectively. In contrast with healthy group, the IDO gene expression and activity were significantly reduced in SVD and 2VD groups (P<0.05). Furthermore, they were conversely associated to secretion of IL6. In conclusion, the data suggested that inflammatory responses in M2 macrophages differentiated from monocytes of patients after treatment of sdLDL may be related to the reduced IDO function.
巨噬细胞是参与改善动脉粥样硬化斑块的重要免疫细胞。M2巨噬细胞有益，因为它能清除血管内皮下空间的无功能成分。在本研究中，我们调查了小而密低密度脂蛋白（sdLDL）对分化的M2巨噬细胞中吲哚胺2,3-双加氧酶（IDO）和白细胞介素（IL6）变化的影响。患者选自接受冠状动脉造影的人群。从健康（狭窄<5%）和患者（狭窄>70%；单支血管病变、双支血管病变和三支血管病变）受试者的全血样本中分离单核细胞，并将其分化为M2巨噬细胞。分别采用RT-qPCR、比色法和ELISA技术检测IDO基因表达、活性和IL6值。与健康组相比，单支血管病变组和双支血管病变组的IDO基因表达和活性显著降低（P<0.05）。此外，它们与IL6的分泌呈负相关。总之，数据表明，sdLDL处理后患者单核细胞分化的M2巨噬细胞中的炎症反应可能与IDO功能降低有关。
