Poursaleh Adeleh, Esfandiari Golnaz, Sadegh Beigee Farahnaz, Eshghifar Nahal, Najafi Mohammad
Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
Shahid Beheshti University of Medical Sciences, Masih Daneshvari Hospital, Tehran, Iran.
Med J Islam Repub Iran. 2019 Jun 4;33:51. doi: 10.34171/mjiri.33.51. eCollection 2019.
Vessel endothelial cells are extensively applied to study the mechanism of atherosclerosis. Some cellular sources including human umbilical artery endothelial cells (HUAECs) and human umbilical vein endothelial cells (HUVECs) are mostly applied in the experimental studies. We described a method for isolating the human endothelial cells from the human thoracic aorta. Normal aortic samples were prepared from subjects with brain death in Masih Daneshvari Hospital. The endothelial cells were isolated using collagenase and were evaluated by the measurement of CD31 marker. Furthermore, the digestion efficacy was studied by vessel histological analysis, and the adhesion mechanism was investigated by leukocyte endothelial adhesion assay kit. The isolation protocol is found as a fast and simple technique with a proper cellular load to separate the endothelial cells from the human aorta.
血管内皮细胞被广泛应用于研究动脉粥样硬化的机制。一些细胞来源，包括人脐动脉内皮细胞（HUAECs）和人脐静脉内皮细胞（HUVECs），大多应用于实验研究。我们描述了一种从人胸主动脉中分离人内皮细胞的方法。正常主动脉样本取自马西赫·达内什瓦里医院脑死亡患者。使用胶原酶分离内皮细胞，并通过测量CD31标志物进行评估。此外，通过血管组织学分析研究消化效果，并通过白细胞内皮细胞粘附检测试剂盒研究粘附机制。该分离方案是一种快速简单的技术，具有合适的细胞载量，可从人主动脉中分离内皮细胞。
