Nikolić Marko Z, Rawlins Emma L
Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN UK.
Curr Pathobiol Rep. 2017;5(2):223-231. doi: 10.1007/s40139-017-0137-7. Epub 2017 Apr 24.
The lung research field has pioneered the use of organoids for the study of cell-cell interactions.
The use of organoids for airway basal cells is routine. However, the development of organoids for the other regions of the lung is still in its infancy. Such cultures usually rely on cell-cell interactions between the stem cells and a putative niche cell for their growth and differentiation.
The use of co-culture organoid systems has facilitated the in vitro cultivation of previously inaccessible stem cell populations, providing a novel method for dissecting the molecular requirements of these cell-cell interactions. Future technology development will allow the growth of epithelial-only organoids in more defined media and also the introduction of specific non-epithelial cells for the study of cell interactions. These developments will require an improved understanding of the epithelial and non-epithelial cell types present in the lung and their lineage relationships.
肺部研究领域率先使用类器官来研究细胞间相互作用。
气道基底细胞类器官的应用已成为常规操作。然而，肺部其他区域类器官的发育仍处于起步阶段。此类培养通常依赖干细胞与假定的龛细胞之间的细胞间相互作用来实现生长和分化。
共培养类器官系统的应用促进了此前难以获取的干细胞群体的体外培养，为剖析这些细胞间相互作用的分子需求提供了一种新方法。未来的技术发展将使仅含上皮细胞的类器官能够在更明确的培养基中生长，还能引入特定的非上皮细胞来研究细胞间相互作用。这些发展需要对肺中存在的上皮和非上皮细胞类型及其谱系关系有更深入的了解。
