Shi Hai-Tao, Shi A-Meng, Wang Yan, Dong Lei, Zhao Gang, Li Yong, Lu Xiao-Lan
Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiao Tong University, Shaanxi Key Laboratory of Gastrointestinal Motility Disorders, Shaanxi Clinical Research Center of Gastrointestinal Disease,Xi'an 710004,China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Sep;47(5):685-690.
To determine the expression of chemokine (C-X-C motif) receptor 7 (CXCR7) in five gastric cancer cell lines with various degrees of differentiation, and the effect of silencing on the migration and invasion of SGC-7901 cells.
The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, SGC-7901, BGC-823 and MKN-28) was detected by Western bolt and RT-PCR. The SGC-7901 cells were transfected with liposome of CXCR7 siRNA to silence gene, and then treated with stromal-derived factor-1 (SDF-1)-the ligand of CXCR7. Transwell assay was used for determining the migratory and invasive ability of SGC-7901 cells in the four groups: NC siRNA, NC siRNA+SDF-1, siRNA and siRNA+SDF-1.
CXCR7 was expressed in the five gastric cancer cell lines, with the highest intensity in SGC-7901. The migrated and invasive cells increased in the NC siRNA+SDF-1 group and reduced in the -siRNA group compared with the NC siRNA group (<0.05). The -siRNA+SDF-1 group had less migrated and invasive cells than the NC siRNA+SDF-1 group (<0.05).
CXCR7 is highly expressed in SGC-7901. SDF-1 promotes the migratory and invasive capability of SGC-7901 cells, but such an effect can be inhibited by silencing it with siRNA.
检测趋化因子（C-X-C基序）受体7（CXCR7）在5种不同分化程度胃癌细胞系中的表达情况，以及沉默CXCR7对SGC-7901细胞迁移和侵袭能力的影响。
采用蛋白质免疫印迹法（Western bolt）和逆转录-聚合酶链反应（RT-PCR）检测胃癌细胞系（HGC-27、MGC-803、SGC-7901、BGC-823和MKN-28）中CXCR7的表达。用CXCR7小干扰RNA（siRNA）脂质体转染SGC-7901细胞以沉默CXCR7基因，然后用CXCR7的配体基质细胞衍生因子-1（SDF-1）处理。采用Transwell实验检测四组SGC-7901细胞的迁移和侵袭能力，四组分别为：阴性对照小干扰RNA（NC siRNA）组、NC siRNA+SDF-1组、CXCR7 siRNA组和CXCR7 siRNA+SDF-1组。
5种胃癌细胞系均有CXCR7表达，其中SGC-7901细胞中CXCR7表达最强。与NC siRNA组相比，NC siRNA+SDF-1组迁移和侵袭细胞数增加，CXCR7 siRNA组迁移和侵袭细胞数减少（P<0.05）。CXCR7 siRNA+SDF-1组迁移和侵袭细胞数少于NC siRNA+SDF-1组（P<0.05）。
CXCR7在SGC-7901细胞中高表达。SDF-1可促进SGC-7901细胞的迁移和侵袭能力，但siRNA沉默CXCR7可抑制该作用。
