基质细胞衍生因子-1/趋化因子受体7轴调控胃癌细胞的增殖、侵袭、黏附和血管生成。
SDF-1/CXCR7 axis regulates the proliferation, invasion, adhesion, and angiogenesis of gastric cancer cells.
作者信息
Ma De-Min, Luo Dian-Xi, Zhang Jie
机构信息
Department of Hepatobiliary and Vascular Surgery, People's Hospital of Dezhou, Dezhou, Shandong Province, 253014, People's Republic of China.
Department of Gastrointestinal Surgery, People's Hospital of Dezhou, 1751 Xin Hu Road, Dezhou, Shandong Province, 253014, People's Republic of China.
出版信息
World J Surg Oncol. 2016 Oct 6;14(1):256. doi: 10.1186/s12957-016-1009-z.
BACKGROUND
More recent studies have revealed that chemokine receptor CXCR7 plays an important role in cancer development. However, little is known about the effect of CXCR7 on the process of gastric cancer cell invasion and angiogenesis. The aim of this study is to investigate the expression of CXCR7 in gastric cancer cell lines and to evaluate the role of CXCR7 in the proliferation, invasion, adhesion, and angiogenesis of gastric cancer cells.
METHODS
Real-time PCR and Western blotting were used to examine the mRNA and protein levels of CXCR4 and CXCR7 in five gastric cancer cell lines (HGC-27, MGC-803, BGC-823, SGC-7901, and MKN-28). CXCR7-expressing shRNA was constructed and subsequently stably transfected into the human gastric cancer cells. In addition, the effect of CXCR7 inhibition on cell proliferation, invasion, adhesion, VEGF secretion, and tube formation was evaluated.
RESULTS
The mRNA and protein of CXCR7 were expressed in all five gastric cancer cell lines; in particular, the expression of CXCR7 was the highest in SGC-7901 cells. Stromal cell-derived factor-1 (SDF-1) was found to induce proliferation, invasion, adhesion, and tube formation. Moreover, the VEGF secretion in SGC-7901 cells was also enhanced by SDF-1 stimulation. These biological effects were inhibited by the silencing of CXCR7 in SGC-7901 cells.
CONCLUSIONS
Increased CXCR7 expression was found in gastric cancer cells. Knockdown of CXCR7 expression by transfection with CXCR7shRNA significantly inhibits SGC-7901 cells' proliferation, invasion, adhesion, and angiogenesis. This study provides new insights into the significance of CXCR7 in the invasion and angiogenesis of gastric cancer.
背景
最近的研究表明趋化因子受体CXCR7在癌症发展中起重要作用。然而，关于CXCR7对胃癌细胞侵袭和血管生成过程的影响知之甚少。本研究的目的是调查CXCR7在胃癌细胞系中的表达，并评估CXCR7在胃癌细胞增殖、侵袭、黏附和血管生成中的作用。
方法
采用实时聚合酶链反应（Real-time PCR）和蛋白质免疫印迹法（Western blotting）检测5种胃癌细胞系（HGC-27、MGC-803、BGC-823、SGC-7901和MKN-28）中CXCR4和CXCR7的mRNA和蛋白水平。构建表达CXCR7的短发夹RNA（shRNA），随后将其稳定转染至人胃癌细胞中。此外，评估CXCR7抑制对细胞增殖、侵袭、黏附、血管内皮生长因子（VEGF）分泌和血管生成的影响。
结果
CXCR7的mRNA和蛋白在所有5种胃癌细胞系中均有表达；特别是，CXCR7在SGC-7901细胞中的表达最高。发现基质细胞衍生因子-1（SDF-1）可诱导增殖、侵袭、黏附和血管生成。此外，SDF-1刺激还可增强SGC-7901细胞中的VEGF分泌。SGC-7901细胞中CXCR7的沉默可抑制这些生物学效应。
结论
在胃癌细胞中发现CXCR7表达增加。用CXCR7 shRNA转染敲低CXCR7表达可显著抑制SGC-7901细胞的增殖、侵袭、黏附和血管生成。本研究为CXCR7在胃癌侵袭和血管生成中的意义提供了新的见解。
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