[脊髓巨噬细胞集落刺激因子及其受体CSF-1R在复杂性区域疼痛综合征I发生发展中的表达]
[Expressions of Spinal Macrophage Colony Stimulating Factor and Its Receptor CSF-1R in the Development ofComplicated Regional Pain Symptom I].
作者信息
Liao Zhi-Min, Tang Yu-Ying, Zheng Yang-Chun, Xu Dan
机构信息
Department of Anesthesiology, West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Disease of Women and Children (Sichuan University), Ministry of Education,Chengdu 610041,China.
Department of Intestinal Surgery, Sichuan Provincial Cancer Hospital,Chengdu 610041,China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Sep;47(5):703-707.
OBJECTIVES
To study the changes of mechanical allodynia and temperature hyperalgesia, as well as the expression of the spinal macrophage colony stimulating factor (M-CSF) and its receptor CSF-1R during the development of complicated regional pain symptom I(CRPS I).
METHODS
The animal model of CRPS I was established using prolonged ischemia-reperfusion injury of rodent left hindpaw. The mechanical allodynia and temperature hyperalgesia of ipsilateral hindpaw were continuously measured for 14 d after reperfusion, and the expressions of spinal M-CSF and CSF-1R in ipsilateral spinal cord horn were measured with immunofluorescence technique on day 3, day 7 and day 14 after reperfusion.
RESULTS
The thresholds of mechanical allodynia and temperature hyperalgesia of ipsilateral hindpaw were significantly decreased (<0.05). M-CSF was secreted by the astrocytes. CSF-1R was primarily distributed on the microglia. The immunofluorescence intensities of M-CSF and CSF-1R in ipsilateral spinal cord horn were significantly increased on day 7 and day 14 after reperfusion (<0.05).
CONCLUSIONS
The ischemia-reperfusion injury simulated pain syndrome in CRPS I and increased the expressions of spinal M-CSF and CSF-1R.
目的
研究复杂区域疼痛综合征I（CRPS I）发展过程中机械性异常性疼痛和温度性痛觉过敏的变化，以及脊髓巨噬细胞集落刺激因子（M-CSF）及其受体CSF-1R的表达。
方法
采用啮齿动物左后爪长时间缺血再灌注损伤建立CRPS I动物模型。再灌注后连续14天测量同侧后爪的机械性异常性疼痛和温度性痛觉过敏，并在再灌注后第3天、第7天和第14天用免疫荧光技术测量同侧脊髓角中脊髓M-CSF和CSF-1R的表达。
结果
同侧后爪的机械性异常性疼痛和温度性痛觉过敏阈值显著降低（<0.05）。M-CSF由星形胶质细胞分泌。CSF-1R主要分布在小胶质细胞上。再灌注后第7天和第14天，同侧脊髓角中M-CSF和CSF-1R的免疫荧光强度显著增加（<0.05）。
结论
缺血再灌注损伤模拟了CRPS I中的疼痛综合征，并增加了脊髓M-CSF和CSF-1R的表达。
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