Luo Bin, Zhang Qian-Wen, Xu Yu, Meng Jian, Wang Xiao-Li, Li Lin, He Yue-Dong
Department of Obstetrics and Gynaecology, West China Second University Hospital, Sichuan University, Chengdu 610041,China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Sep;47(5):718-721.
To study the effect of high mobility group chromosomal protein N2 on inhibiting cervical cancer in nude mice.
Model of cervical cancer were established in nude mice. They were randomly divided into 4 groups including negative control group, HMGN2 group, cisplatin group and HMGN2 with cisplatin group. After 4 injections, the tumor size were calculated and tumor tissues were stained by haematoxylin eosin (HE) staining.
Transplated tumor models were established successfully. The tumor sizes of negative control group [(0.38±0.12) cm³] were significantly lower than those of HMGN2 group [(0.14±0.07)cm³, cisplatin group [(0.11±0.06) cm³] and HMGN2 combined with cisplatin group[(0.11±0.07) cm³]. No differences were detected in HMGN2 group, cisplatin group and HMGN2 with cisplatin group in tumor sizes. The tumor inhibition rates of HMGN2 group, cisplatin group and HMGN2 with cisplatin group were 0.62±0.18, 0.71±0.17 and 0.70±0.18, respectively. The necrosis area were smaller in negative control group than in other three groups by HE staining.
HMGN2 has a significant inhibitory effect on transplanted cervical cancer in nude mice.
研究高迁移率族染色体蛋白N2对裸鼠宫颈癌的抑制作用。
建立裸鼠宫颈癌模型。将其随机分为4组，包括阴性对照组、HMGN2组、顺铂组和HMGN2联合顺铂组。注射4次后，计算肿瘤大小，并用苏木精-伊红（HE）染色法对肿瘤组织进行染色。
成功建立移植瘤模型。阴性对照组的肿瘤大小[（0.38±0.12）cm³]显著低于HMGN2组[（0.14±0.07）cm³]、顺铂组[（0.11±0.06）cm³]和HMGN2联合顺铂组[（0.11±0.07）cm³]。HMGN2组、顺铂组和HMGN2联合顺铂组的肿瘤大小无差异。HMGN2组、顺铂组和HMGN2联合顺铂组的肿瘤抑制率分别为0.62±0.18、0.71±0.17和0.70±0.18。HE染色显示阴性对照组的坏死面积小于其他三组。
HMGN2对裸鼠移植性宫颈癌有显著抑制作用。
