Storck Katharina, Fischer Reyk, Buchberger Maria, Haller Bernhard, Regn Sybille
Ear, Nose, and Throat, Head and Neck Surgery Department, Technical University of Munich, Munich, Germany.
Institute of Medical Statistics and Epidemiology , Technical University of Munich, Munich, Germany.
Laryngoscope. 2017 Dec;127(12):E428-E436. doi: 10.1002/lary.26694. Epub 2017 Jun 9.
OBJECTIVES/HYPOTHESIS: Adipose tissue engineering aims to provide functional tissue surrogates for the restoration of soft tissue defects and contour deformities in the face. Many studies involve the delivery of cells; however, the impact and the exact role of the implanted cells is not yet fully elucidated.
Animal research.
In this study, we used a mouse model for the development of volume-stable adipose tissue using polyurethane scaffolds combined with a long-term stable fibrin gel and adipose-derived stromal cells to investigate the influence of cell delivery on tissue development.
After 12 weeks in vivo, the emerging tissue in these constructs was shown to be exclusively of host origin by human-specific vimentin staining. Comparison of unseeded versus seeded scaffolds revealed a significant effect of the delivered cells on adipose tissue development as shown by histological staining and histomorphometric quantification of adipocytes, whereas blood vessel formation was not affected by delivery of adipose-derived stromal cells at this time point.
This is evidence for an indirect action of the implanted cells, providing a proadipogenic microenvironment within constructs, which was further boosted by adipogenic precultivation of the seeded constructs. Especially in peripheral areas of the constructs, the number of adipocytes was significantly elevated in seeded scaffolds compared to nonseeded controls, suggesting that the implanted cells likely triggered the invasion and differentiation of host cells. This is supported by the fact that the provision of a fat rich environment (by coverage of the constructs with a fat flap upon implantation) additionally stimulated adipose tissue formation.
NA. Laryngoscope, 127:E428-E436, 2017.
目的/假设：脂肪组织工程旨在提供功能性组织替代物，以修复面部软组织缺损和轮廓畸形。许多研究涉及细胞递送；然而，植入细胞的影响和确切作用尚未完全阐明。
动物研究。
在本研究中，我们使用小鼠模型，通过将聚氨酯支架与长期稳定的纤维蛋白凝胶和脂肪来源的基质细胞相结合，来构建体积稳定的脂肪组织，以研究细胞递送对组织发育的影响。
体内培养12周后，通过人特异性波形蛋白染色显示，这些构建体中出现的组织完全来自宿主。未接种与接种支架的比较显示，如组织学染色和脂肪细胞的组织形态计量学定量所示，递送的细胞对脂肪组织发育有显著影响，而在这个时间点，脂肪来源的基质细胞递送对血管形成没有影响。
这证明了植入细胞的间接作用，即在构建体内提供促脂肪生成的微环境，而接种构建体的脂肪生成预培养进一步增强了这种微环境。特别是在构建体的周边区域，与未接种的对照相比，接种支架中的脂肪细胞数量显著增加，这表明植入细胞可能触发了宿主细胞的侵入和分化。植入时用脂肪瓣覆盖构建体以提供富含脂肪的环境会额外刺激脂肪组织形成，这一事实支持了上述观点。
无。《喉镜》，2017年，第127卷，E428 - E436页。
