Griessl Michael, Buchberger Anna-Maria, Regn Sybille, Kreutzer Kilian, Storck Katharina
Department of ENT, Head and Neck Surgery, Technical University of Munich, Munich, Germany.
Department of Maxillofacial Surgery, University Clinic of the Charité Berlin, Berlin, Germany.
Laryngoscope. 2018 Jun;128(6):E206-E213. doi: 10.1002/lary.27124. Epub 2018 Feb 15.
OBJECTIVES/HYPOTHESIS: To find an alternative approach to contemporary techniques in tissue augmentation and reconstruction, tissue engineering strategies aim to involve adipose-derived stem and stromal cells (ASCs) harboring a strong differentiation potential into various tissue types such as bone, cartilage, and fat.
Animal research.
The stromal vascular fraction (SVF) was used directly as a cell source to provide a potential alternative to contemporary ASC-based adipose tissue engineering. Seeded in TissuCol fibrin, we applied ASCs or SVF cells to porous, degradable polyurethane (PU) scaffolds.
We successfully demonstrated the in vivo generation of volume-stable, well-vascularized PU-based constructs containing host-derived mature fat pads. Seeded human stem cells served as modulators of host-cell migration rather than differentiating themselves. We further demonstrated that preliminary culture of SVF cells was not necessary.
Our results bring adipose tissue engineering, together with automated processing devices, closer to clinical applicability. The time-consuming and cost-intensive culture and induction of the ASCs is not necessary.
NA. Laryngoscope, 128:E206-E213, 2018.
目的/假设：为了找到一种替代当代组织增大和重建技术的方法，组织工程策略旨在利用具有强大分化潜能的脂肪来源的干细胞和基质细胞（ASC），使其分化为各种组织类型，如骨、软骨和脂肪。
动物研究。
直接使用基质血管成分（SVF）作为细胞来源，为当代基于ASC的脂肪组织工程提供一种潜在的替代方法。将ASC或SVF细胞接种到TissuCol纤维蛋白中，然后应用于多孔、可降解的聚氨酯（PU）支架。
我们成功地证明了在体内生成了体积稳定、血管化良好的基于PU的构建体，其中包含宿主来源的成熟脂肪垫。接种的人类干细胞充当宿主细胞迁移的调节因子，而不是自身分化。我们进一步证明，SVF细胞无需进行预培养。
我们的结果使脂肪组织工程与自动化处理设备一起更接近临床应用。无需对ASC进行耗时且成本高昂的培养和诱导。
无。《喉镜》，2018年，第128卷，E206 - E213页。
