Latino Maria Agnese, Botta Giovanni, Badino Claudia, Maria Daniela De, Petrozziello Annalisa, Sensini Alessandra, Leli Christian
Department of Laboratory Medicine, Unit of Bacteriology, Città della Salute e della Scienza, Turin, Italy.
Fetal and Maternal Pathology, Department of Pathology, OIRM/Sant' Anna, Città della Salute e della Scienza, Turin, Italy.
J Perinat Med. 2018 Jul 26;46(5):503-508. doi: 10.1515/jpm-2016-0305.
Ureaplasma parvum, Ureaplasma urealyticum and Mycoplasma hominis are also known as genital mycoplasmas. Acute chorioamnionitis is an inflammation of the placenta associated with miscarriage. We retrospectively evaluated a possible association between genital mycoplasmas detection, acute chorioamnionitis and fetal pneumonia from second and third trimester spontaneous abortions.
One hundred and thirty placenta and fetal lung samples were evaluated for histological examination. The placenta samples, along with corresponding fetal tracheo-bronchial aspirates, also underwent bacterial and fungal culture and real-time polymerase chain reaction (PCR) assay for the detection of genital mycoplasmas.
Acute chorioamnionitis and pneumonia were diagnosed in 80/130 (61.5%) and 22/130 (16.9%) samples, respectively. Among samples positive for acute chorioamnionitis, the proportion of samples positive by real-time PCR and/or culture, was significantly higher than that of negative controls [54/80 (67.5%) vs. 26/80 (32.5%); P<0.001]. Ureaplasma parvum detection was significantly associated with acute chorioamnionitis compared to controls [9/11 (81.8%) vs. 2/11 (18.2%); P=0.019], as well as U. urealyticum [6/7 (85.7%) vs. 1/7 (14.3%); P=0.039]. Among tracheo-bronchial aspirates from abortions with pneumonia, the proportion of real-time PCR and/or culture positive samples was significantly higher than that of controls [13/22 (59.1%) vs. 9/22 (40.9%); P=0.029].
A strong association was found between acute histologic chorioamnionitis and microbial invasion with U. parvum and/or U. urealyticum.
微小脲原体、解脲脲原体和人型支原体也被称为生殖支原体。急性绒毛膜羊膜炎是一种与流产相关的胎盘炎症。我们回顾性评估了生殖支原体检测、急性绒毛膜羊膜炎与孕中期和孕晚期自然流产所致胎儿肺炎之间的可能关联。
对130份胎盘和胎儿肺样本进行组织学检查评估。胎盘样本以及相应的胎儿气管支气管吸出物，还进行了细菌和真菌培养以及用于检测生殖支原体的实时聚合酶链反应（PCR）分析。
分别在80/130（61.5%）和22/130（16.9%）的样本中诊断出急性绒毛膜羊膜炎和肺炎。在急性绒毛膜羊膜炎阳性的样本中，实时PCR和/或培养阳性样本的比例显著高于阴性对照[54/80（67.5%）对26/80（32.5%）；P<0.001]。与对照相比，微小脲原体检测与急性绒毛膜羊膜炎显著相关[9/11（81.8%）对2/11（18.2%）；P=0.019]，解脲脲原体也是如此[6/7（85.7%）对1/7（14.3%）；P=0.039]。在患有肺炎的流产胎儿的气管支气管吸出物中，实时PCR和/或培养阳性样本的比例显著高于对照[13/22（59.1%）对9/22（40.9%）；P=0.029]。
发现急性组织学绒毛膜羊膜炎与微小脲原体和/或解脲脲原体的微生物入侵之间存在密切关联。
