Kim Seung-Hyun, Park Hyun-Jung, Moon Dong-Oh
Department of Biology Education, Daegu University, Gyeongsan, Gyeongsang 38453, Republic of Korea.
Oncol Lett. 2017 Jun;13(6):4427-4432. doi: 10.3892/ol.2017.5950. Epub 2017 Mar 29.
Sulforaphane (SFN), an isothiocyanate present in cruciferous vegetables, has been demonstrated to inhibit the growth of various types of cancer cell. The aim of the present study was to investigate whether SFN sensitizes breast cancer cells to paclitaxel-induced apoptosis and to identify the signal pathway through which SFN mediates apoptosis. Combined treatment of breast cancer cells with SFN and paclitaxel resulted in increased activation of apoptotic signaling pathway members, including caspase-3, -8 and -9, and cytochrome c, compared with treatment with SFN or paclitaxel alone. In addition, treatment with SFN and paclitaxel resulted in downregulation of the nuclear factor kappa B signaling pathway, and reduced protein expression of apoptosis regulator Bcl-2 and phosphorylated AKT serine/threonine kinase. Furthermore, SFN-paclitaxel-induced apoptosis was inhibited by overexpression of Bcl-2. The results of the present study suggest that combined treatment with SFN and paclitaxel is a novel therapeutic strategy for the treatment of breast cancer.
萝卜硫素（SFN）是十字花科蔬菜中的一种异硫氰酸盐，已被证明能抑制多种类型癌细胞的生长。本研究的目的是调查SFN是否能使乳腺癌细胞对紫杉醇诱导的凋亡敏感，并确定SFN介导凋亡的信号通路。与单独使用SFN或紫杉醇处理相比，用SFN和紫杉醇联合处理乳腺癌细胞导致凋亡信号通路成员（包括半胱天冬酶-3、-8和-9以及细胞色素c）的激活增加。此外，用SFN和紫杉醇处理导致核因子κB信号通路下调，并降低凋亡调节因子Bcl-2和磷酸化AKT丝氨酸/苏氨酸激酶的蛋白表达。此外，Bcl-2的过表达抑制了SFN-紫杉醇诱导的凋亡。本研究结果表明，SFN和紫杉醇联合处理是治疗乳腺癌的一种新的治疗策略。
