小鼠肠道微生物群改变与胆固醇胆结石形成的关联
Alteration of gut microbiota in association with cholesterol gallstone formation in mice.
作者信息
Wang Qihan, Jiao Long, He Chuanqi, Sun Haidong, Cai Qu, Han Tianquan, Hu Hai
机构信息
Department of Surgery, Shanghai Institute of Digestive Surgery Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 200025, Shanghai, China.
Institute of Gallstone Disease, Center of Gallbladder Disease, Shanghai East Hospital, Tongji University School of Medicine, #150 Jimo Road, Shanghai, 201200, China.
出版信息
BMC Gastroenterol. 2017 Jun 9;17(1):74. doi: 10.1186/s12876-017-0629-2.
BACKGROUND
The gut microbiome exerts extensive roles in metabolism of nutrients, pharmaceuticals, organic chemicals. Little has been known for the role of gut microbiota in regulating cholesterol and bile acids in association with gallstone formation. This study investigated the changes in the composition of gut microbiota in mice fed with lithogenic diet (LD).
METHODS
Adult male C57BL/6 J mice were fed with either lithogenic diet (1.25% cholesterol and 0.5% cholic acid) or chow diet as control for 56 days. The fecal microbiota were determined by 16S rRNA gene sequencing.
RESULTS
LD led to formation of cholesterol gallstone in mice. The richness and alpha diversity of gut microbial reduced in mice fed with LD. Firmicutes was significantly decreased from 59.71% under chow diet to 31.45% under LD, P < 0.01, as well as the ratio of Firmicutes to Bacteroidetes. Differences in gut microbiota composition were also observed at phylum, family and genus levels between the two groups.
CONCLUSION
Our results suggested that gut microbiota dysbiosis might play an important role in the pathogenesis of cholesterol gallstone formation in mice.
背景
肠道微生物群在营养物质、药物和有机化学品的代谢中发挥着广泛作用。关于肠道微生物群在与胆结石形成相关的胆固醇和胆汁酸调节中的作用，人们了解甚少。本研究调查了喂食致石饮食（LD）的小鼠肠道微生物群组成的变化。
方法
成年雄性C57BL/6 J小鼠分别喂食致石饮食（1.25%胆固醇和0.5%胆酸）或普通饮食作为对照，持续56天。通过16S rRNA基因测序确定粪便微生物群。
结果
LD导致小鼠形成胆固醇结石。喂食LD的小鼠肠道微生物的丰富度和α多样性降低。厚壁菌门从普通饮食组的59.71%显著降至LD组的31.45%，P < 0.01，厚壁菌门与拟杆菌门的比例也降低。两组在门、科和属水平上也观察到肠道微生物群组成的差异。
结论
我们的结果表明，肠道微生物群失调可能在小鼠胆固醇结石形成的发病机制中起重要作用。
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