Cordero Oscar J, Varela-Calviño Rubén, López-González Tania, Grujic Milica, Juranic Zorica, Mouriño Coral, Hernández-Rodríguez Íñigo, Rodríguez-López Marina, de la Iglesia Bruno Aspe, Pego-Reigosa José María
Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Clin Biochem. 2017 Nov;50(16-17):903-910. doi: 10.1016/j.clinbiochem.2017.06.001. Epub 2017 Jun 6.
Rheumatoid arthritis (RA) patients show low serum levels of the Ag dipeptidyl peptidase IV (DPP-IV/CD26), both soluble CD26 (sCD26) concentration and its DPP-IV activity. The aim of this study was to test if anti-DPP-IV/CD26 Abs (Anti-CD26) cleared sCD26.
DESIGN & METHODS: Serum Anti-CD26 and Total titers (as comparison) of isotypes IgA, IgM and IgG as well as sCD26 concentration and DPP-IV activity were measured in a cohort of RA patients undergoing different biological and non-biological therapies (n=105) and controls (n=50).
Anti-CD26 levels were increased approximately two-fold for each isotype in RA, were not related to the sCD26 clearance, showed several correlations with disease activity parameters, were significantly higher in smokers and they were not ACPA. Anti-CD26 Igs showed high diagnostic power (82% sensitivity and 96% specificity) and their levels differed amongst the different groups of patients stratified by the type of therapy.
As DPP-IV/CD26 is associated to factors triggering RA in the lung and periodontal tissue, these results suggest that Anti-CD26 isotypes may participate in pathogenesis and may be useful as biomarkers for earlier diagnosis and/or precision medicine.
类风湿关节炎（RA）患者血清中Ag二肽基肽酶IV（DPP-IV/CD26）水平较低，包括可溶性CD26（sCD26）浓度及其DPP-IV活性。本研究的目的是测试抗DPP-IV/CD26抗体（抗CD26）是否能清除sCD26。
在一组接受不同生物和非生物疗法的RA患者（n = 105）和对照组（n = 50）中，测量血清抗CD26和各亚型IgA、IgM和IgG的总滴度（作为对照）以及sCD26浓度和DPP-IV活性。
RA患者中各亚型的抗CD26水平增加了约两倍，与sCD26清除无关，与疾病活动参数有多种相关性，吸烟者中显著更高，且不是抗环瓜氨酸肽抗体（ACPA）。抗CD26免疫球蛋白显示出较高的诊断能力（82%的敏感性和96%的特异性），其水平在按治疗类型分层的不同患者组中有所不同。
由于DPP-IV/CD26与引发肺部和牙周组织中RA的因素相关，这些结果表明抗CD26亚型可能参与发病机制，并且可能作为早期诊断和/或精准医学的生物标志物。
