核磁共振在大小RNA结构测定中的应用。
Applications of NMR to structure determination of RNAs large and small.
作者信息
Barnwal Ravi P, Yang Fan, Varani Gabriele
机构信息
Department of Chemistry, University of Washington, Seattle 98195, WA, USA.
Department of Chemistry, University of Washington, Seattle 98195, WA, USA.
出版信息
Arch Biochem Biophys. 2017 Aug 15;628:42-56. doi: 10.1016/j.abb.2017.06.003. Epub 2017 Jun 16.
Abstract
Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool to investigate the structure and dynamics of RNA, because many biologically important RNAs have conformationally flexible structures, which makes them difficult to crystallize. Functional, independently folded RNA domains, range in size between simple stem-loops of as few as 10-20 nucleotides, to 50-70 nucleotides, the size of tRNA and many small ribozymes, to a few hundred nucleotides, the size of more complex RNA enzymes and of the functional domains of non-coding transcripts. In this review, we discuss new methods for sample preparation, assignment strategies and structure determination for independently folded RNA domains of up to 100 kDa in molecular weight.
摘要
核磁共振（NMR）光谱是研究RNA结构和动力学的有力工具，因为许多具有生物学重要性的RNA具有构象灵活的结构，这使得它们难以结晶。功能性、独立折叠的RNA结构域，其大小范围从仅有10 - 20个核苷酸的简单茎环，到50 - 70个核苷酸（tRNA和许多小核酶的大小），再到几百个核苷酸（更复杂的RNA酶和非编码转录本功能结构域的大小）。在本综述中，我们讨论了分子量高达100 kDa的独立折叠RNA结构域的样品制备、归属策略和结构测定的新方法。
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