慢性炎症性神经病中针对结旁和结周抗原的自身抗体反应。
Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies.
作者信息
Mathey E K, Garg N, Park S B, Nguyen T, Baker S, Yuki N, Yiannikas C, Lin C S, Spies J M, Ghaoui R, Barnett M H, Vucic S, Pollard J D, Kiernan M C
机构信息
Brain & Mind Centre, University of Sydney, Sydney, Australia.
Brain & Mind Centre, University of Sydney, Sydney, Australia.
出版信息
J Neuroimmunol. 2017 Aug 15;309:41-46. doi: 10.1016/j.jneuroim.2017.05.002. Epub 2017 May 13.
Autoantibodies to nodal/paranodal proteins have been reported in patients with chronic inflammatory demyelinating polyneuropathy (CIDP) and multifocal motor neuropathy (MMN). To determine the frequency of anti-paranodal antibodies in our cohort of CIDP patients and to validate the presence anti-nodal antibodies in MMN, sera were screened for IgG against human neurofascin 155, contactin-1, neurofascin 186 and gliomedin using ELISA. In CIDP patients, 7% were anti-NF155 IgG positive and 7% were anti-CNTN1 IgG positive. Positive results were confirmed using cell based assays and indirect immunofluorescence on teased nerve fibres. We did not detect IgG autoantibodies against these nodal/paranodal antigens in MMN patients.
慢性炎性脱髓鞘性多发性神经病（CIDP）和多灶性运动神经病（MMN）患者中已报告存在针对结旁/结周蛋白的自身抗体。为了确定我们队列中CIDP患者抗结周抗体的频率，并验证MMN中抗结抗体的存在，我们使用酶联免疫吸附测定（ELISA）法筛查血清中针对人神经束蛋白155、接触蛋白-1、神经束蛋白186和神经胶质瘤蛋白的IgG。在CIDP患者中，7%的患者抗NF155 IgG呈阳性，7%的患者抗CNTN1 IgG呈阳性。通过基于细胞的检测方法和对 teased 神经纤维进行间接免疫荧光检测，对阳性结果进行了确认。在MMN患者中，我们未检测到针对这些结旁/结周抗原的IgG自身抗体。
Zotero 插件