Talukdar B, Kalita H K, Basumatary S, Saikia D J, Sarma D
Department of Zoology, Gauhati University, Guwahati 781014, Assam, India.
Department of Zoology, Gauhati University, Guwahati 781014, Assam, India.
Ecotoxicol Environ Saf. 2017 Oct;144:72-78. doi: 10.1016/j.ecoenv.2017.06.007. Epub 2017 Jun 8.
The investigation deals with the effects of Acid Mine Drainage (AMD) of coal mine on fish Channa punctata (Bloch) by examining the incidence of haematological, morphological, histological changes and DNA fragmentation in tissues of C. punctata in laboratory condition. For this study fishes were exposed to 10% of AMD for a period of 30 days. The fusion of the primary and secondary gill lamellae, distortion, loss of alignment, deposition of worn out tissues and mucous on the surface of the lamella in the gills; degeneration of morphological architecture, loss of alignment of tubules, mucous deposition in the kidney; cellular damage, cellular necrosis, extraneous deposition on the surface, pore formation in the liver are some important changes detected by scanning electron microscopy. Fishes of AMD treated group showed gradual significant decrease in TEC, Hb and, increase in TLC and DLC as compared to that of the control. DNA fragmentation observed in kidney of fishes from treated group indicates an intricate pollutant present in the AMD. The high incidence of morphological and histological alterations, haematological changes along with DNA breakage in C. punctata is an evidence of the cytotoxic and genotoxic potential of AMD of coal mines.
本研究通过在实验室条件下检测斑点叉尾鮰（Channa punctata，Bloch）组织中的血液学、形态学、组织学变化及DNA片段化发生率，探讨煤矿酸性矿井水（AMD）对斑点叉尾鮰的影响。在本研究中，将鱼类暴露于10%的AMD中30天。扫描电子显微镜检测到的一些重要变化包括：鳃小片的初级和次级鳃小片融合、变形、排列紊乱、鳃小片表面磨损组织和黏液沉积；肾脏形态结构退化、肾小管排列紊乱、黏液沉积；肝脏细胞损伤、细胞坏死、表面异物沉积、形成孔隙。与对照组相比，AMD处理组的鱼类红细胞计数（TEC）、血红蛋白（Hb）逐渐显著降低，白细胞计数（TLC）和淋巴细胞计数（DLC）增加。处理组鱼类肾脏中观察到的DNA片段化表明AMD中存在一种复杂的污染物。斑点叉尾鮰形态学和组织学改变、血液学变化以及DNA断裂的高发生率，证明了煤矿AMD具有细胞毒性和基因毒性潜力。
