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本地微生物群对未水解蘑菇种植废料进行厌氧产氢

Anaerobic hydrogen production from unhydrolyzed mushroom farm waste by indigenous microbiota.

作者信息

Lin Chiu-Yue, Lay Chyi-How, Sung I-Yuan, Sen Biswarup, Chen Chin-Chao

机构信息

Green Energy Technology Research Group, Ton Duc Thang University, 19 Nguyen Huu Tho, District No. 7, Ho Chi Minh City 700000, Viet Nam; Faculty of Environment and Labor Safety, Ton Duc Thang University, 19 Nguyen Huu Tho, District No. 7, Ho Chi Minh City 700000, Viet Nam.

Green Energy Technology Research Group, Ton Duc Thang University, 19 Nguyen Huu Tho, District No. 7, Ho Chi Minh City 700000, Viet Nam; Green Energy Development Center, Feng Chia University, No. 100, Wenhwa Rd., Seatwen, Taichung 40724, Taiwan.

出版信息

J Biosci Bioeng. 2017 Oct;124(4):425-429. doi: 10.1016/j.jbiosc.2017.05.001. Epub 2017 Jun 7.

DOI:10.1016/j.jbiosc.2017.05.001
PMID:28601607
Abstract

The cultivation of mushrooms generates large amounts of waste polypropylene bags stuffed with wood flour and bacterial nutrients that makes the mushroom waste (MW) a potential feedstock for anaerobic bioH fermentation. MW indigenous bacteria were enriched using thermophilic temperature (55°C) for use as the seed inoculum without any external seeding. The peak hydrogen production rate (6.84 mmol H/L-d) was obtained with cultivation pH 8 and substrate concentration of 60 g MW/L in batch fermentation. Hydrogen production yield (HY) is pH and substrate concentration dependent with an HY decline occurring at pH and substrate concentration increasing from pH 8 to 10 and 60 to 80 g MW/L, respectively. The fermentation bioH production from MW is in an acetate-type metabolic path.

摘要

蘑菇栽培会产生大量装满木粉和细菌营养物质的废弃聚丙烯袋,这使得蘑菇废料(MW)成为厌氧生物制氢发酵的潜在原料。利用嗜热温度(55°C)富集MW中的本土细菌,用作种子接种物,无需任何外部接种。在分批发酵中,培养pH值为8且底物浓度为60 g MW/L时,获得了最高产氢率(6.84 mmol H/L-d)。产氢量(HY)取决于pH值和底物浓度,当pH值从8增加到10以及底物浓度从60 g MW/L增加到80 g MW/L时,HY分别下降。MW发酵生物制氢遵循乙酸型代谢途径。

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