Yildirim Nuri, Akman Levent, Acar Kamuran, Demir Sibel, Ozkan Sultan, Alan Nuran, Zekioglu Osman, Terek M Cosan, Ozdemir Necmettin, Ozsaran Aydin
Ege University, Faculty of Medicine, Department of Obstetrics and Gynecology, Izmir, Turkey.
Ege University, Faculty of Medicine, Department of Obstetrics and Gynecology, Izmir, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2017 Aug;215:55-61. doi: 10.1016/j.ejogrb.2017.06.005. Epub 2017 Jun 4.
The aim of this study is to evaluate the impact of lymphocyte infiltration on prognostic parameters, recurrence and survival in ovarian cancer.
Sixty-two patients who were primarily operated for epithelial ovarian carcinoma between 1997 and 2008 were included. CD3, CD4, CD8, CD20 and FoxP3 expressions were evaluated immunohistochemically on sections obtained from paraffin-embedded tissues.
Median follow up was 87 months. In whole cohort, CD3+ and CD8+ T lymphocyte infiltrations were significantly higher in patients with high-grade tumors, advanced stage tumors and the patients with omental metastasis (for CD3 p=0.0001, p=0.029, p=0.016; for CD8 p=0.044, p=0.002, p=0.046, respectively). DFS was significantly lower among patients with CD8+ T lymphocytes with regard to patients who did not have CD8+ T lymphocyte infiltration (p=0.028). In univariate analysis, presence of CD8 cytotoxic T lymphocyte infiltration (p=0.03), stage (0.0001), tumor grade (p=0.007), omental metastasis (p=0.0001) and lymph node metastasis (p=0.0001) were significant risk factors for recurrence. But in multivariate analysis, only stage [HR: 116.6 (95% CI: 13.09-1039.45) (p=0.0001)] was found as an independent risk factor for recurrence.
CD3+ and CD8+ T lymphocyte infiltrations were related with advanced stage, high-grade tumor and the omental metastasis in ovarian cancer. DFS was significantly shorter in patients with CD8+ T lymphocyte infiltration. CD3+ and CD8+ T lymphocyte infiltrations were related with poor prognosis in ovarian cancer.
本研究旨在评估淋巴细胞浸润对卵巢癌预后参数、复发及生存的影响。
纳入1997年至2008年间因上皮性卵巢癌接受初次手术的62例患者。对石蜡包埋组织切片进行免疫组织化学评估CD3、CD4、CD8、CD20和FoxP3的表达。
中位随访时间为87个月。在整个队列中，高级别肿瘤、晚期肿瘤及有网膜转移的患者中CD3⁺和CD8⁺T淋巴细胞浸润显著更高（CD3分别为p = 0.0001、p = 0.029、p = 0.016；CD8分别为p = 0.044、p = 0.002、p = 0.046）。与无CD8⁺T淋巴细胞浸润的患者相比，有CD8⁺T淋巴细胞浸润的患者无病生存期显著更低（p = 0.028）。单因素分析中，CD8细胞毒性T淋巴细胞浸润（p = 0.03）、分期（0.0001）、肿瘤分级（p = 0.007）、网膜转移（p = 0.0001）和淋巴结转移（p = 0.0001）是复发的显著危险因素。但多因素分析中，仅分期[风险比：116.6（95%置信区间：13.09 - 1039.45）（p = 0.0001）]被发现是复发的独立危险因素。
CD3⁺和CD8⁺T淋巴细胞浸润与卵巢癌的晚期、高级别肿瘤及网膜转移相关。有CD8⁺T淋巴细胞浸润的患者无病生存期显著更短。CD3⁺和CD8⁺T淋巴细胞浸润与卵巢癌预后不良相关。
