State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China.
Bioresour Technol. 2017 Oct;241:573-581. doi: 10.1016/j.biortech.2017.05.157. Epub 2017 May 29.
Three anaerobic digestion (AD) processes of waste activated sludge (WAS) were established including the control (mono-WAS), one-stage AD and two-stage AD along with microwave-HO pre-treatment (MW-HO) to investigate the profiles and drivers of antibiotic resistance genes (ARGs) distribution concerning co-selection from heavy metals, intI1 and microbial community through qPCR and high-throughput sequencing method. Results showed that MW-HO could reduce the absolute gene copies of all ARGs while increased the relative abundance of most ARGs. After subsequent AD, both total ARGs quantities and relative abundance were enriched while two-stage AD showed some advantages over ARGs abundance reduction. Besides, AD was more effective on the potential pathogens reduction than MW-HO. AD could reduce the role of intI1 on the spread of ARGs, while mantel test and procrustes analysis indicated that the variation of ARGs abundance was closely associated with the discrepancy of bacterial community.
建立了三种厌氧消化（AD）工艺，包括对照（单-WAS）、单级 AD 和两级 AD 以及微波-HO 预处理（MW-HO），以通过 qPCR 和高通量测序方法研究与重金属、intI1 和微生物群落的共选择有关的抗生素抗性基因（ARGs）分布的特征和驱动因素。结果表明，MW-HO 可以减少所有 ARGs 的绝对基因拷贝数，同时增加大多数 ARGs 的相对丰度。随后进行 AD 后，总 ARGs 数量和相对丰度都得到了富集，而两级 AD 在减少 ARGs 丰度方面表现出一些优势。此外，AD 对潜在病原体的减少比 MW-HO 更有效。AD 可以降低 intI1 对 ARGs 传播的作用，而 Mantel 测试和 Procrustes 分析表明，ARGs 丰度的变化与细菌群落的差异密切相关。
