TU Braunschweig, Braunschweig, Germany.
Bioprocess Biosyst Eng. 2017 Sep;40(9):1399-1410. doi: 10.1007/s00449-017-1797-8. Epub 2017 Jun 10.
A hybrid-immobilization method was developed to improve the long-term stability of laminaribiose phosphorylase immobilized on epoxy supports Sepabeads EC-EP/S. Entrapment in chitosan retained all of the enzyme activity depending on the amount of entrapped solid materials and increased half-life by a factor of 10-94.4 h. No enzyme activity loss was determined during 12 times reuse. The immobilization method is also applicable to sucrose phosphorylase immobilized on Sepabeads EC-EP/S. Up to 31.9 g/L laminaribiose were produced in bienzymatic batch experiments with reaction-integrated product separation by adsorption on zeolites.
开发了一种混合固定化方法，以提高层粘连糖磷酸化酶在环氧载体 Sepabeads EC-EP/S 上的长期稳定性。壳聚糖包埋保留了所有的酶活性，这取决于包埋的固体材料的量，并使半衰期提高了 10-94.4 h。在 12 次重复使用中没有确定酶活性损失。该固定化方法也适用于蔗糖磷酸化酶在 Sepabeads EC-EP/S 上的固定化。在通过沸石吸附进行反应整合产物分离的双酶批实验中，可生产高达 31.9 g/L 的层粘连糖。
