Abi Akram, Hartig Dave, Vorländer Karl, Wang Anqi, Scholl Stephan, Jördening Hans-Joachim
Institute for Technical Chemistry Technische Universität Braunschweig Braunschweig Germany.
Institute for Chemical and Thermal Process Engineering Technische Universität Braunschweig Braunschweig Germany.
Eng Life Sci. 2018 Oct 4;19(1):4-12. doi: 10.1002/elsc.201800110. eCollection 2019 Jan.
Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g g in the desorbate with a total productivity of 5.6 mg L h without the use of recycles.
将由蔗糖和葡萄糖双酶法生产纤维二糖与在BEA沸石上的吸附相结合，引入了第一步捕获和纯化步骤。在毫升规模的分批模式下，对包括洗涤和解吸步骤在内的下游加工进行了表征和优化。然后将结果转移到填充床系统中进行酶促生产和吸附，评估了吸附剂粒径对纯度和生产率的影响。最后，连续10天进行纤维二糖的酶促生产。负载沸石的后续下游加工在解吸液中产生了超过0.5 g/g的纯度，总生产率为5.6 mg L⁻¹ h⁻¹，且无需使用循环。
