Ma Bomou, Sun Qisong, Yang Jing, Wizi Jakpa, Hou Xiuliang, Yang Yiqi
Key Laboratory of Eco-Textiles, Ministry of Education, College of textile and clothing, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Department of Textiles, Merchandising & Fashion Design, University of Nebraska-Lincoln, 234, HECO Building, Lincoln, NE, 68583-0802, USA.
Environ Sci Pollut Res Int. 2017 Jul;24(21):17711-17718. doi: 10.1007/s11356-017-9410-x. Epub 2017 Jun 10.
Chicken feather, a potential source of keratin, is often disposed as waste material. Although some methods, i.e., hydrolysis, reduction, and oxidation, have been developed to isolate keratin for composites, it has been limited due to the rising environmental concerns. In this work, a green solvent N-methylmorpholine N-oxide (NMMO) was used to extract keratin from chicken feather waste. Eighty-nine percent of keratin was extracted using 75% NMMO solution. However, the result from size exclusion HPLC showed that most of the keratin degraded into polypeptide with molecular weight of 2189 and only 25.3% regenerated keratin was obtained with molecular weight of 14,485. Analysis of amino acid composition showed a severe damage to the disulfide bonds in keratin during the extraction procedure. Oxidization had an important effect on the reconstitution of the disulfide bonds, which formed a stable three-dimensional net structure in the regenerated keratins. Besides, Raman spectra, NMR, FT-IR, XRD, and TGA were used to characterize the properties of regenerated keratin and raw chicken feather. In the end, a possible mechanism was proposed based on the results.
鸡毛作为角蛋白的潜在来源，常被当作废料处理。尽管已经开发了一些方法，如水解、还原和氧化，来从鸡毛中分离角蛋白用于复合材料，但由于环境问题日益受到关注，这些方法受到了限制。在这项工作中，一种绿色溶剂N-甲基吗啉N-氧化物（NMMO）被用于从鸡毛废料中提取角蛋白。使用75%的NMMO溶液提取了89%的角蛋白。然而，尺寸排阻高效液相色谱的结果表明，大部分角蛋白降解为分子量为2189的多肽，仅获得了25.3%的分子量为14485的再生角蛋白。氨基酸组成分析表明，在提取过程中角蛋白中的二硫键受到了严重破坏。氧化对二硫键的重构有重要影响，在再生角蛋白中形成了稳定的三维网络结构。此外，拉曼光谱、核磁共振、傅里叶变换红外光谱、X射线衍射和热重分析被用于表征再生角蛋白和生鸡毛的性能。最后，根据结果提出了一种可能的机制。
