Chao Ran, Mishra Shekhar, Si Tong, Zhao Huimin
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Metab Eng. 2017 Jul;42:98-108. doi: 10.1016/j.ymben.2017.06.003. Epub 2017 Jun 7.
Engineered biological systems such as genetic circuits and microbial cell factories have promised to solve many challenges in the modern society. However, the artisanal processes of research and development are slow, expensive, and inconsistent, representing a major obstacle in biotechnology and bioengineering. In recent years, biological foundries or biofoundries have been developed to automate design-build-test engineering cycles in an effort to accelerate these processes. This review summarizes the enabling technologies for such biofoundries as well as their early successes and remaining challenges.
诸如基因电路和微生物细胞工厂等工程化生物系统有望解决现代社会中的许多挑战。然而,研发的手工流程缓慢、昂贵且不一致,这是生物技术和生物工程中的一个主要障碍。近年来,生物铸造厂或生物工厂已被开发出来,以实现设计-构建-测试工程周期的自动化,从而加速这些流程。本综述总结了此类生物铸造厂的使能技术及其早期成功案例和仍存在的挑战。