Jang Yeoung-Hwan, Subramanian Dharaneedharan, Won Seung-Hwan, Heo Moon-Soo
Jeju Special Self-Governing Province Ocean and Fisheries Research Institute, Pyoseon-myeon, Segwipo-si, Jeju 697-914, South Korea.
Marine Pathogenic Microbes and Aquatic Disease Control Lab, School of Marine Life Sciences, Jeju National University, Jeju 690-756, South Korea.
Fish Shellfish Immunol. 2017 Aug;67:172-178. doi: 10.1016/j.fsi.2017.06.019. Epub 2017 Jun 7.
This study evaluated the pathophysiological, biochemical, and immunological status of olive flounder (Paralichthys olivaceus) infected with the myxosporean parasite Kudoa septempunctata. Flounder fish collected from Kudoa-infected and uninfected farms were confirmed by microscopic and TaqMan probe-based quantitative PCR screening. Morphological, biochemical, histological, and immune gene expression analyses were performed on uninfected and infected hosts to assess the effect of K. septempunctata. Histological studies confirmed the presence of Kudoa myxospores in the trunk muscles of infected flounder fish. Serum biochemical parameters, including the levels of myeloperoxidase activity, superoxide dismutase activity, alanine aminotransferase, alkaline phosphatase, amylase, bilirubin, total protein, cholesterol, calcium, potassium, sodium, phosphorus, glucose, and galactose, were found to exhibit no significant variations (p > 0.05) between uninfected and infected flounder fish. However, immune-related genes such as Mx, lysozyme, signal transducer and activator of transcription 1, interferon-γ, interferon regulatory factor, and tumour necrosis factor showed significantly elevated expression (p < 0.05) in the trunk muscles of infected flounder fish while no significant differences were noted in uninfected fish trunk muscle and head-kidney of infected and uninfected flounder fish.
本研究评估了感染粘孢子虫库道虫（Kudoa septempunctata）的牙鲆（Paralichthys olivaceus）的病理生理、生化和免疫状态。通过显微镜检查和基于TaqMan探针的定量PCR筛选，对从受库道虫感染和未感染的养殖场采集的牙鲆进行了确认。对未感染和感染的宿主进行了形态学、生化、组织学和免疫基因表达分析，以评估库道虫的影响。组织学研究证实，在受感染牙鲆的躯干肌肉中存在库道虫粘孢子。血清生化参数，包括髓过氧化物酶活性、超氧化物歧化酶活性、丙氨酸转氨酶、碱性磷酸酶、淀粉酶、胆红素、总蛋白、胆固醇、钙、钾、钠、磷、葡萄糖和半乳糖水平，在未感染和感染的牙鲆之间未发现显著差异（p>0.05）。然而，免疫相关基因，如Mx、溶菌酶、信号转导和转录激活因子1、干扰素-γ、干扰素调节因子和肿瘤坏死因子，在受感染牙鲆的躯干肌肉中表达显著升高（p<0.05），而在未感染牙鲆的躯干肌肉以及受感染和未感染牙鲆的头肾中未发现显著差异。
