Vlahović Milena, Matić Dragana, Mutić Jelena, Trifković Jelena, Đurđić Slađana, Perić Mataruga Vesna
University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Insect Physiology and Biochemistry, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
University of Belgrade, Institute for Biological Research "Siniša Stanković", Department of Insect Physiology and Biochemistry, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
Comp Biochem Physiol C Toxicol Pharmacol. 2017 Oct;200:27-33. doi: 10.1016/j.cbpc.2017.06.003. Epub 2017 Jun 8.
Bioaccumulation and excretion of heavy metals in insects is only partially clarified. We have investigated cadmium accumulation in the feces, head and integument of Lymantria dispar (Lepidoptera: Lymantriidae) larvae exposed to chronic dietary intake of cadmium. The aim of the experiment was to establish modalities of metal accumulation, primarily cadmium, as well as changes in fitness traits in two insect populations receiving 50 or 100μgCd/g dry food. The egg-masses originated from two localities: a protected nature reserve (unpolluted population) and an area near a busy highway (polluted population) in Serbia. At both added dietary levels cadmium concentration was highest in feces. Small alterations in metal concentrations after both cadmium treatments were detected in the integument. It was established that irrespective of population origin, the modality of decline of larval mass and relative growth rate (RGR) was similar. Concentrations of Cu and Zn in the integument were approximately the same regardless of dietary cadmium intake. However, cadmium accumulation in the examined tissues, as well as variability of fitness traits depended on population origin and cadmium concentration. In larvae from both populations not given contaminated food the head was the organ with the greatest accumulation of trace elements. Our results reveal how invasive phytophagous insects cope with high metal concentrations in their food by body mass (RGR) reduction and energy allocation towards processes that enable accumulation of cadmium and other trace elements in different tissues.
昆虫体内重金属的生物累积和排泄情况仅得到部分阐明。我们研究了长期通过饮食摄入镉的舞毒蛾（鳞翅目：毒蛾科）幼虫粪便、头部和体表中的镉积累情况。该实验的目的是确定金属积累的方式，主要是镉的积累方式，以及接受50或100μgCd/g干粮的两个昆虫种群的适合度性状变化。卵块来自塞尔维亚的两个地方：一个受保护的自然保护区（未受污染种群）和一条繁忙高速公路附近的区域（受污染种群）。在两种添加的饮食水平下，粪便中的镉浓度最高。在两种镉处理后，体表中的金属浓度有微小变化。已确定，无论种群来源如何，幼虫体重和相对生长率（RGR）的下降方式相似。无论饮食中镉的摄入量如何，体表中铜和锌的浓度大致相同。然而，所检查组织中的镉积累以及适合度性状的变异性取决于种群来源和镉浓度。在两个未喂食受污染食物的种群的幼虫中，头部是微量元素积累最多的器官。我们的研究结果揭示了入侵性植食性昆虫如何通过降低体重（RGR）以及将能量分配到使镉和其他微量元素在不同组织中积累的过程来应对食物中高浓度的金属。
