Blixt Frank W, Radziwon-Balicka Aneta, Edvinsson Lars, Warfvinge Karin
Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden.
Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet, Glostrup, Denmark.
Exp Eye Res. 2017 Aug;161:124-131. doi: 10.1016/j.exer.2017.06.002. Epub 2017 Jun 8.
Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.
降钙素基因相关肽（CGRP）是一种由37个氨基酸组成的神经肽，具有多种功能，包括血管舒张、痛觉刺激感知和炎症反应。CGRP受体由两个主要成分组成：降钙素样受体（CLR）和受体活性修饰蛋白1（RAMP1）。虽然人们越来越认识到CGRP在偏头痛中起关键作用，但CGRP在视网膜中的功能尚未完全明确。本研究旨在通过免疫组织化学方法直观地以及使用流式细胞术定量研究CGRP及其两种受体成分在大鼠视网膜中的分布。在穆勒细胞中发现了CGRP免疫反应性，而CLR/RAMP1位于神经纤维层。此外，由于几乎所有RAMP1免疫反应性细胞都共表达CLR，我们认为视网膜中RAMP1的表达反映了功能性CGRP受体。
