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中国北方河北省农村腹泻儿童中诺如病毒的变异分析

Variation analysis of norovirus among children with diarrhea in rural Hebei Province, north of China.

作者信息

Qiao Niu, Wang Song-Mei, Wang Jin-Xia, Kang Bin, Zhen Shan-Shan, Zhang Xin-Jiang, Hao Zhi-Yong, Ma Jing-Chen, Qiu Chao, Zhao Yu-Liang, Liu Lei, Wang Xuan-Yi

机构信息

Key Laboratory of Medical Molecular Virology of MoE & MoH, Fudan University, Shanghai 200032, People's Republic of China; Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, People's Republic of China.

Laboratory of Molecular Biology, Training Center of Medical Experiments, School of Basic Medical Sciences, Fudan University, Shanghai 200032, People's Republic of China.

出版信息

Infect Genet Evol. 2017 Sep;53:199-205. doi: 10.1016/j.meegid.2017.06.007. Epub 2017 Jun 7.

Abstract

To understand the distribution of genotyping, as well as evolution of norovirus circulating among children<5yrs., a population-based diarrhea surveillance targeted children<5yrs. was conducted in rural Zhengding County, Hebei Province, China between October 2011 and March 2012. RT-PCR was used to amplify the capsid-encoding region of GI and GII norovirus to identify norovirus infection. All PCR products were sequenced and analyzed for genotyping and constructing phylogenetic tree. Dynamic distribution network was constructed by TempNet to illustrate the genetic relationships at two different time points. Bayesian evolutionary inference techniques were applied by BEAST software to study the norovirus evolution rate. During the 6-month surveillance period, 1091 episodes of diarrhea were reported from 5633 children under 5years of age lived in catchment area. 115 of 1091 stool specimens were detected as norovirus positive (10.54%). Five genotypes based on capsid gene sequences were identified, including GII.2 (11), GII.3 (52), GII.4 (47), GII.6 (4) and GII.7 (1). An identical haplotype of GII.4 circulated between 2006 and 2011 in Hebei Province. A mean rate of 6.29×10 nucleotide substitutions/site/year (s/s/y) was obtained for GII.3 viruses in Hebei, while the GII.4 viruses evolved at a mean rate of 3.67×10s/s/y. In conclusions, GII.3 (45.22%) and GII. 4(40.87%) are the predominant strain in Hebei Province in the winter season of 2011 and 2012. Different from the current consensus, our study shows that GII.3 noroviruses in Hebei Province evolved at a faster rate than GII.4 viruses.

摘要

为了解5岁以下儿童中诺如病毒的基因分型分布及流行情况,2011年10月至2012年3月在中国河北省正定县农村地区开展了一项针对5岁以下儿童的基于人群的腹泻监测。采用逆转录聚合酶链反应(RT-PCR)扩增GI和GII型诺如病毒的衣壳编码区,以鉴定诺如病毒感染。对所有PCR产物进行测序,并分析其基因分型和构建系统发育树。通过TempNet构建动态分布网络,以阐明两个不同时间点的遗传关系。应用贝叶斯进化推理技术,通过BEAST软件研究诺如病毒的进化速率。在6个月的监测期内,集水区内5633名5岁以下儿童报告了1091例腹泻病例。1091份粪便标本中有115份检测为诺如病毒阳性(10.54%)。根据衣壳基因序列鉴定出5种基因型,包括GII.2(11株)、GII.3(52株)、GII.4(47株)、GII.6(4株)和GII.7(1株)。一种相同的GII.4单倍型在2006年至2011年期间在河北省流行。河北省GII.3病毒的平均进化速率为6.29×10核苷酸替换/位点/年(s/s/y),而GII.4病毒的平均进化速率为3.67×10s/s/y。结论是,GII.3(45.22%)和GII.4(40.87%)是2011年和2012年冬季河北省的主要毒株。与目前的共识不同,我们的研究表明,河北省的GII.3诺如病毒进化速率比GII.4病毒更快。

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