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中国婴幼儿有症状和无症状诺如病毒感染的分子流行病学分析。

Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants.

机构信息

Key Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan University, 138 Yi Xue Yuan Rd., Shanghai, 200032, People's Republic of China.

Laboratory of Molecular Biology, Training Center of Medical Experiments, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, People's Republic of China.

出版信息

Virol J. 2023 Apr 4;20(1):60. doi: 10.1186/s12985-023-02024-z.

DOI:10.1186/s12985-023-02024-z
PMID:37016444
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10074819/
Abstract

BACKGROUND

Norovirus is a leading cause of acute gastroenteritis among children. Previous studies based on symptomatic infections indicated that mutations, rather than recombination drove the evolution of the norovirus ORF2. These characteristics were found in hospital-based symptomatic infections, whereas, asymptomatic infections are frequent and contribute significantly to transmission.

METHODS

We conducted the first norovirus molecular epidemiology analysis covering both symptomatic and asymptomatic infections derived from a birth cohort study in the northern China.

RESULTS

During the study, 14 symptomatic and 20 asymptomatic norovirus infections were detected in 32 infants. Out of the 14 strains that caused symptomatic infections, 12 strains were identified as GII.3[P12], and others were GII.4[P31]. Conversely, 17 asymptomatic infections were caused by GII.4[P31], two by GII.2[P16], and one by GII.4[P16]. Regardless of symptomatic and asymptomatic infections, the mutations were detected frequently in the ORF2 region, and almost all recombination were identified in the RdRp-ORF2 region. The majority of the mutations were located around the predefined epitope regions of P2 subdomain indicating a potential for immune evasion.

CONCLUSION

The role of symptomatic as well as asymptomatic infections in the evolution of norovirus needs to be evaluated continuously.

摘要

背景

诺如病毒是导致儿童急性肠胃炎的主要原因。基于有症状感染的先前研究表明，突变而非重组推动了诺如病毒 ORF2 的进化。这些特征在基于医院的有症状感染中被发现，而无症状感染则很常见，并对传播有重大贡献。

方法

我们进行了首次诺如病毒分子流行病学分析，涵盖了来自中国北方一个出生队列研究的有症状和无症状感染。

结果

在研究期间，在 32 名婴儿中检测到 14 例有症状和 20 例无症状的诺如病毒感染。在导致有症状感染的 14 株病毒中，有 12 株鉴定为 GII.3[P12]，其余为 GII.4[P31]。相反，17 例无症状感染由 GII.4[P31]引起，2 例由 GII.2[P16]引起，1 例由 GII.4[P16]引起。无论是否有症状和无症状感染，ORF2 区域都经常检测到突变，几乎所有重组都发生在 RdRp-ORF2 区域。大多数突变位于 P2 亚结构域的预定义表位区域附近，表明存在免疫逃避的可能性。

结论

需要不断评估有症状和无症状感染在诺如病毒进化中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/642c0d3708ba/12985_2023_2024_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/71a58b1fdb75/12985_2023_2024_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/2f653db71d7a/12985_2023_2024_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/2d9bd743c992/12985_2023_2024_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/10486e0d09cc/12985_2023_2024_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/642c0d3708ba/12985_2023_2024_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/71a58b1fdb75/12985_2023_2024_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/2f653db71d7a/12985_2023_2024_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/2d9bd743c992/12985_2023_2024_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/10486e0d09cc/12985_2023_2024_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5107/10074819/642c0d3708ba/12985_2023_2024_Fig5_HTML.jpg

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