Dara Mahintaj, Khashei Reza, Dehghani Behzad
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine; Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Infez Med. 2017 Jun 1;25(2):123-126.
Different virulence factors are involved in Helicobacter pylori pathogenesis. H. pylori outer membrane proteins are a family of virulence factors that have diverse members. HopQ (H. pylori outer membrane protein) is the largest of them that contains types I and II alleles. The role of hopQ is not exactly known, but it has been considered in H. pylori adhesion and colonization. The aim of this study was to determine the frequency of hopQ genotypes among H. pylori isolates obtained from patients with gastroduodenal disorders and their association with the clinical outcome. The DNA of 100 H. pylori clinical isolates was investigated by polymerase chain reaction (PCR) method using specific primers for determining the hopQI and hopQII genotypes. hopQI was present in 35%, while hopQII was positive in 55% of the isolates. Amongst the gastritis subjects, the rate of hopQII compared to hopQI was higher, and a statistically significant difference was found between hopQII genotype and the clinical outcome. With respect to the significant difference between the hopQ genotype and clinical outcome in our clinical isolates, it seems that this genotype is a useful marker for evaluating its association with H. pylori-related diseases.
不同的毒力因子参与幽门螺杆菌的致病过程。幽门螺杆菌外膜蛋白是一类具有多种成员的毒力因子家族。HopQ（幽门螺杆菌外膜蛋白）是其中最大的一种，包含I型和II型等位基因。HopQ的作用尚不完全清楚，但它被认为与幽门螺杆菌的黏附和定植有关。本研究的目的是确定从胃十二指肠疾病患者中分离出的幽门螺杆菌菌株中hopQ基因型的频率及其与临床结果的关联。使用用于确定hopQI和hopQII基因型的特异性引物，通过聚合酶链反应（PCR）方法对100株幽门螺杆菌临床分离株的DNA进行了研究。35%的分离株中存在hopQI，而55%的分离株中hopQII呈阳性。在胃炎患者中，hopQII与hopQI相比的比例更高，并且在hopQII基因型与临床结果之间发现了统计学上的显著差异。鉴于我们临床分离株中hopQ基因型与临床结果之间存在显著差异，这种基因型似乎是评估其与幽门螺杆菌相关疾病关联的有用标志物。
