Kazemi E, Kahrizi D, Moradi M T, Sohrabi M, Yari K
Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
Razi University Department of Agronomy and Plant Breeding Kermanshah Iran
Cell Mol Biol (Noisy-le-grand). 2016 Feb 29;62(2):107-10.
The Helicobacter pylori is a Gram-negative, microaerophilic bacterium found usually in the stomach and use a number of mechanisms to survive in the stomach lumen. The presence of these bacteria in the stomach can lead to gastritis and reduction in stomach acid production. Acute inflammation can directly damage to the peripheral cells that are responsible for the secretion of acid. The risk of developing gastric carcinoma is associated to heterogeneity of Helicobacter pylori virulence factors. The HopQII is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also play a role in the virulence of H. pylori. The purpose of the current study was to investigate the association between different H. pylori virulence hopQII allele and patients with gastroduodenal disorders. For this purpose 58 stomach biopsies of patients with gastric cancer and 100 saliva samples from healthy individuals were collected. Then genomic DNA was purified and PCR for was done for desired genes via specific primers. The H. pylori infections were diagnosed by PCR for GlmM gene. Then frequencies of hopQII+ and hopQII- genotypes was determined in H. pylori infected cases. Statistical analysis showed that there were not significant differences between healthy and diseased ones for genotype hopQII+.
幽门螺杆菌是一种革兰氏阴性、微需氧细菌，通常存在于胃中，并利用多种机制在胃腔中存活。胃中存在这些细菌会导致胃炎并减少胃酸分泌。急性炎症可直接损害负责分泌胃酸的外周细胞。患胃癌的风险与幽门螺杆菌毒力因子的异质性有关。HopQII是参与细菌粘附胃黏膜的外膜蛋白之一，有人认为它在幽门螺杆菌的毒力中也起作用。本研究的目的是调查不同幽门螺杆菌毒力hopQII等位基因与胃十二指肠疾病患者之间的关联。为此，收集了58例胃癌患者的胃活检组织和100例健康个体的唾液样本。然后纯化基因组DNA，并通过特异性引物对所需基因进行PCR扩增。通过针对GlnM基因的PCR诊断幽门螺杆菌感染。然后在幽门螺杆菌感染病例中确定hopQII+和hopQII-基因型的频率。统计分析表明，hopQII+基因型在健康人和患病者之间没有显著差异。
