Chiu Yu-Jui, Cai Wei, Lee Tiffany, Kraimer Julia, Lo Yu-Hwa
Materials Science and Engineering Program, University of California at San Diego La Jolla, California, USA.
Radiological Health Engineering Program, Texas A & M University, Texas, USA.
Bio Protoc. 2017 Feb 20;7(4). doi: 10.21769/BioProtoc.2143.
To study the inhomogeneity within a cell population including exosomes properties such as exosome secretion rate of cells and surface markers carried by exosomes, we need to quantify and characterize those exosomes secreted by each individual cell. Here we develop a method to collect and analyze exosomes secreted by an array of single cells using antibody-modified glass slides that are position-registered to each single cell. After each collection, anti-body conjugated quantum dots are used to label exosomes to allow counting and analysis of exosome surface proteins. Detailed studies of exosome properties related to cell behaviors such as responses to drugs and stress at single cell resolution can be found in the publication (Chiu ., 2016).
为了研究细胞群体内的异质性，包括外泌体特性，如细胞的外泌体分泌率和外泌体携带的表面标志物，我们需要对每个单个细胞分泌的外泌体进行定量和表征。在这里，我们开发了一种方法，使用与每个单个细胞位置配准的抗体修饰玻片来收集和分析由一系列单个细胞分泌的外泌体。每次收集后，使用抗体偶联量子点标记外泌体，以便对外泌体表面蛋白进行计数和分析。有关外泌体特性与细胞行为（如单细胞分辨率下对药物和应激的反应）相关的详细研究可在参考文献（Chiu等，2016）中找到。
