Labas Valérie, Teixeira-Gomes Ana-Paula, Bouguereau Laura, Gargaros Audrey, Spina Lucie, Marestaing Aurélie, Uzbekova Svetlana
UMR PRC, INRA 85, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France.
Data Brief. 2017 May 26;13:175-179. doi: 10.1016/j.dib.2017.05.042. eCollection 2017 Aug.
The endogenous peptides and small proteins extracted from bovine ovarian follicular cells (oocytes, cumulus and granulosa cells) were identified by Top-down High Resolution Mass Spectrometry (TD-HR-MS/MS) in order to annotate peptido- and proteoforms detected using qualitative and quantitative profiling method based on ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The description and analysis of these Top-down MS data in the context of oocyte quality biomarkers research are available in the original research article of Labas et al. (2017) http://dx.doi.org/10.1016/j.jprot.2017.03.027[1]. Raw data derived from this peptidomic/proteomic analysis have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (dataset identifier PXD004892). Here, we described the inventory of all identified peptido- and proteoforms including their biochemical and structural features, and functional annotation of correspondent proteins. This peptide/protein inventory revealed that TD-HR-MS/MS was appropriate method for both global and targeted proteomic analysis of ovarian tissues, and it can be further employed as a reference for other studies on follicular cells including single oocytes.
为了注释使用基于完整细胞基质辅助激光解吸/电离飞行时间质谱（ICM-MS）的定性和定量分析方法检测到的肽和蛋白质形式，采用自上而下的高分辨率质谱（TD-HR-MS/MS）对从牛卵巢卵泡细胞（卵母细胞、卵丘细胞和颗粒细胞）中提取的内源性肽和小蛋白质进行了鉴定。在卵母细胞质量生物标志物研究背景下对这些自上而下的质谱数据的描述和分析，可在Labas等人（2017年）的原始研究文章中获取，网址为http://dx.doi.org/10.1016/j.jprot.2017.03.027[1]。源自该肽组学/蛋白质组学分析的原始数据已通过PRIDE合作伙伴存储库存入蛋白质组交换联盟（数据集标识符PXD004892）。在此，我们描述了所有已鉴定的肽和蛋白质形式的清单，包括它们的生化和结构特征以及相应蛋白质的功能注释。该肽/蛋白质清单表明，TD-HR-MS/MS是卵巢组织整体和靶向蛋白质组学分析的合适方法，并且可进一步用作包括单个卵母细胞在内的卵泡细胞其他研究的参考。
