Biological & Clinical Research Department, L'Oreal Research and Innovation, Aulnay sous Bois, France.
Exp Dermatol. 2018 May;27(5):460-462. doi: 10.1111/exd.13390. Epub 2017 Aug 14.
Keratinocytes of the basal layer function are to maintain tissue homoeostasis and to fulfil skin repair in response to an external aggression. In wound-healing, during re-epithelialization phase, epithelial precursor cells gradually migrate from the edges of the wound. The epidermal reconstruction model called standard model allows the vertical skin regeneration process (proliferation/differentiation) to being investigated, and keratinocyte function in preserving skin homoeostasis to being assessed. Here, we developed and characterized a 3D migration model, which introduces a step of keratinocytes migration such as the one observed in the phase of re-epithelialization in wound-healing process. We validated the added value and the discriminative potential of this model by demonstrating pro-epithelializing effects of compounds. This new model allows the role of keratinocytes in different biomechanical and environmental requests to being better understood, and brings a new tool for compound screening and the study of mechanisms involved in skin regeneration.
基底层的角质形成细胞的功能是维持组织内稳态，并在受到外部侵袭时进行皮肤修复。在伤口愈合过程中，在再上皮化阶段，上皮前体细胞逐渐从伤口边缘迁移。表皮重建模型称为标准模型，它允许研究垂直皮肤再生过程（增殖/分化），并评估角质形成细胞在维持皮肤内稳态方面的功能。在这里，我们开发并表征了一种 3D 迁移模型，该模型引入了角质形成细胞迁移的步骤，如在伤口愈合过程中再上皮化阶段观察到的那样。我们通过证明化合物的促上皮化作用验证了该模型的附加值和区分潜力。这种新模型可以更好地理解角质形成细胞在不同生物力学和环境要求下的作用，并为化合物筛选和研究皮肤再生涉及的机制提供了新工具。
