Hou Lin, Wang Wei, Wang Mei-Kun, Song Xue-Song
Department of Anesthesiology, The First Hospital of Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2022 Apr 25;10:878588. doi: 10.3389/fbioe.2022.878588. eCollection 2022.
Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity and and could effectively inhibit the growth of and . Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.
皮肤伤口愈合是一项重要的临床挑战，主要治疗要点是加速表皮再生和预防感染。因此，有必要开发一种能同时治愈细菌感染并加速伤口愈合的伤口敷料。在此，我们报道一种负载壳聚糖（CS）结合碱性成纤维细胞生长因子（CSBD-bFGF）和抗菌肽（P5S9K）的多功能复合伤口敷料。首先，将CS用作敷料基质材料，并将P5S9K包裹在CS中。然后，通过结合重组DNA技术和酪氨酸酶处理设计CSBD-bFGF，并在敷料材料表面进行修饰。结果表明，与市售bFGF相比，CSBD-bFGF与CS的结合能力显著提高，且CSBD-bFGF可从CS敷料中可控释放。更重要的是，制备的敷料材料显示出优异的抗菌活性，能够有效抑制[具体细菌名称]和[具体细菌名称]的生长。以NIH3T3细胞作为细胞模型，结果表明CSBD-bFGF@CS/P5S9K复合敷料是一种对细胞生长友好的材料。细胞接种在复合敷料表面后，细胞中胶原蛋白-1（COL-1）和血管内皮生长因子（VEGF）基因表达显著上调。最后，应用大鼠背部全层伤口模型分析复合敷料的组织修复能力。结果表明，含有CSBD-bFGF和P5S9K的复合敷料具有最强的皮肤伤口修复能力。因此，CSBD-bFGF@CS/P5S9K复合敷料具有良好的抗菌和加速伤口愈合能力，在皮肤伤口治疗中具有良好的应用前景。
