Wang Hye-Young, Uh Young, Kim Seoyong, Lee Hyeyoung
Optipharm, Inc., Wonju Eco Environmental Technology Center, Wonju, Gangwon, 26493, Republic of Korea.
Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, Gangwon, 26426, Republic of Korea.
J Med Microbiol. 2017 Jun;66(6):777-787. doi: 10.1099/jmm.0.000495. Epub 2017 Jun 13.
The purpose of this study was to evaluate the usefulness of a new diagnostic multiplexed bead-based bioassay (Quantamatrix Multiplexed Assay Platform; QMAP) system with shape-encoded silica microparticles for the rapid and accurate detection and identification of 23 mycobacterial species/groups, including Mycobacterium tuberculosis complex (MTBC).
A total of 295 mycobacterial clinical isolates cultured from respiratory specimens were used for identification of MTBC and non-tuberculous mycobacteria (NTM) using the QMAP system and the results were confirmed with PCR-restriction fragment length polymorphism (RFLP) analysis of the rpoB gene, rpoB sequence analysis and PCR-reverse blot hybridization assay (REBA).Results/Key findings. The Mycobacterium genus-specific probe of the QMAP system was positive for all 46 Mycobacterium reference strains and negative for 59 non-Mycobacterium strains. Based on 295 liquid culture-positive samples, both the culture-based conventional identification method and the QMAP system identified each 212 and 81 isolates as MTB and NTM species. The concordance rates for the identification of NTM species between the QMAP system and molecular assays were 92.8 % (77/83), 97.6 % (81/83) and 100 % (83/83) for PCR-RFLP, the rpoB sequence analysis and PCR-REBA, respectively.
The QMAP system yielded rapid, highly sensitive and specific results for the identification of MTBC and NTM and accurately discriminated between NTM species within 3 h.
本研究旨在评估一种基于形状编码二氧化硅微粒的新型诊断多重微珠生物测定系统（Quantamatrix多重测定平台；QMAP）用于快速、准确检测和鉴定包括结核分枝杆菌复合群（MTBC）在内的23种分枝杆菌菌种/菌群的实用性。
从呼吸道标本中培养的295株分枝杆菌临床分离株用于使用QMAP系统鉴定MTBC和非结核分枝杆菌（NTM），结果通过rpoB基因的PCR-限制性片段长度多态性（RFLP）分析、rpoB序列分析和PCR-反向印迹杂交测定（REBA）进行确认。结果/主要发现。QMAP系统的分枝杆菌属特异性探针对所有46株分枝杆菌参考菌株呈阳性，对59株非分枝杆菌菌株呈阴性。基于295份液体培养阳性样本，基于培养的传统鉴定方法和QMAP系统分别将212株和81株分离株鉴定为结核分枝杆菌和非结核分枝杆菌菌种。QMAP系统与分子测定法之间鉴定非结核分枝杆菌菌种的一致率分别为PCR-RFLP的92.8%（77/83）、rpoB序列分析的97.6%（81/83）和PCR-REBA的100%（83/83）。
QMAP系统在鉴定MTBC和NTM方面产生了快速、高度敏感和特异的结果，并在3小时内准确区分了非结核分枝杆菌菌种。
