Department of Anesthesiology, Affiliated Drum Tower Hospital of Medical School of Nanjing University, Nanjing, Jiangsu, China.
Department of Anesthesiology, The First People's Hospital of Lianyungang City, Lianyungang, China.
Spine (Phila Pa 1976). 2018 Feb 1;43(3):E126-E131. doi: 10.1097/BRS.0000000000002279.
Experimental study.
To evaluate the relationship between clip occlusal depth and functional and histological outcome measures in a rat model of thoracic spinal cord injury (SCI).
Aneurysm clip compression is a proven model of contusion-compression SCI, but the relationship between clip depth and outcomes in thoracic SCI is unknown.
A single aneurysm clip was applied to the spinal cord at thoracic vertebra 10 for 1 minute with an occlusal depth of 2, 6, or 10 mm. The actual compression force was measured using a self-made pulling method. Locomotor function was assessed for 28 days using Basso, Beattie, and Bresnahan (BBB) and inclined plane test (IPT) scores. We then used hematoxylin-eosin and Luxol fast blue staining to histologically quantify cavitation formation, preserved white matter, and preserved grey matter.
Greater occlusal compression depths caused greater actual compression forces and worsened functional and histological recovery. The 2- and 10-mm clip injury groups had significantly different BBB and ITP scores; cavitation, preserved white matter, and preserved grey matter volumes; and actual force measures (P < 0.05).
Our findings show that the occlusal depth of clip compression correlates with actual compression force and recovery impairment.
实验研究。
在大鼠胸段脊髓损伤（SCI）模型中，评估夹闭咬合深度与功能和组织学结果指标之间的关系。
动脉瘤夹压迫是一种已被证实的挫伤-压迫性 SCI 模型，但胸 SCI 中夹闭深度与结果之间的关系尚不清楚。
用单个动脉瘤夹在 T10 胸椎的脊髓上夹闭 1 分钟，夹闭深度为 2、6 或 10mm。使用自制的牵拉法测量实际的压缩力。使用 Basso、Beattie 和 Bresnahan（BBB）和斜板试验（IPT）评分在 28 天内评估运动功能。然后使用苏木精-伊红和卢索快速蓝染色对空洞形成、保留的白质和保留的灰质进行组织学定量。
更大的咬合压缩深度导致更大的实际压缩力，并使功能和组织学恢复恶化。2mm 和 10mm 夹伤组的 BBB 和 ITP 评分、空洞、保留的白质和保留的灰质体积以及实际力测量值有显著差异（P<0.05）。
我们的研究结果表明，夹闭咬合深度与实际压缩力和恢复损伤程度相关。
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