Powder Technology Laboratory, Institute of Materials, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Analyst. 2017 Jun 26;142(13):2338-2342. doi: 10.1039/c7an00382j.
We developed a method to correct absorbance-based toxicity tests to remove the influence of the deposited dose of nanoparticles. As a model of absorbance-based toxicity tests, we chose the frequently used MTS test. The corrected cell viabilities obtained with the MTS test matched the results of the fluorescence-based PrestoBlue® test.
我们开发了一种方法来修正基于吸光度的毒性测试,以去除纳米颗粒沉积剂量的影响。作为基于吸光度的毒性测试的模型,我们选择了常用的 MTS 测试。用 MTS 测试修正后的细胞活力与基于荧光的 PrestoBlue®测试的结果相匹配。