Department of Materials Meet Life, Swiss Federal Laboratories for Materials Science and Technology (Empa), Lerchenfeldstrasse 5, 9014, St Gallen, Switzerland.
Nanoscale. 2017 Jun 22;9(24):8418-8426. doi: 10.1039/c7nr01176h.
Despite decades of research, wound complications remain a major cause of postoperative mortality, especially in the face of multiple comorbidities. Addressing the issue of anastomotic leakages and impaired wound healing from a new angle is of great interest with the prospect of having direct impact on patient outcome. Recently, aqueous suspensions of silica and iron oxide nanoparticles have been employed to connect biological tissue by serving as an adhesive layer eventually leading to macroscopic gluing of tissue. In this work, we explore the prospects of this effect by introducing bioactive tissue adhesives composed of nanoparticles produced via scalable and sterile flame spray pyrolysis. We investigate six different metal oxides on cytocompatibility, hemostatic activity and adhesive properties in a small intestine lap joint model. While bioglass nanoparticles show exceptionally strong procoagulant and adhesive properties, the cell membrane integrity is impaired at high particle concentrations. Interestingly, when bioglass is combined with ceria, a material that has well-documented cytoprotective effects, the resulting hybrid particles exhibit the same beneficiary effects as bioglass while featuring superior cytocompatibility. Taken together, we demonstrate highly modular synthesis of nanoparticles expressing adhesive properties in conjunction with tailored bioactivity. Such bioactive nanoparticles as adhesion nuclei in wound healing have a wide range of potential applications in surgical wound care and regenerative medicine.
尽管经过了几十年的研究，伤口并发症仍然是术后死亡的主要原因，尤其是在面临多种合并症的情况下。从新的角度解决吻合口漏和伤口愈合受损的问题非常有趣，因为这有可能直接影响患者的预后。最近，已经使用二氧化硅和氧化铁纳米粒子的水悬浮液来连接生物组织，充当粘合层，最终导致组织的宏观粘合。在这项工作中，我们通过引入通过可扩展和无菌火焰喷雾热解产生的纳米颗粒制成的生物活性组织粘合剂来探索这种效果的前景。我们在小肠搭接模型中研究了六种不同的金属氧化物对细胞相容性、止血活性和粘合性能的影响。虽然生物玻璃纳米颗粒表现出异常强的促凝和粘合性能，但在高浓度的颗粒下，细胞膜的完整性受到损害。有趣的是，当生物玻璃与具有良好记载的细胞保护作用的氧化铈结合时，所得的混合颗粒表现出与生物玻璃相同的有益效果，同时具有更好的细胞相容性。总之，我们证明了具有粘合性能的纳米颗粒的高度模块化合成与定制的生物活性相结合。作为伤口愈合中的粘合核的这种生物活性纳米颗粒在外科伤口护理和再生医学中有广泛的潜在应用。
