Key Laboratory of Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
Key Laboratory of Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
J Colloid Interface Sci. 2022 Feb;607(Pt 2):1239-1252. doi: 10.1016/j.jcis.2021.09.092. Epub 2021 Sep 20.
Tissue adhesives have received much attention for their effectiveness in sealing wounds or incisions in clinical surgery, especially in minimally invasive surgery. To meet the safe and smart wound management requirements, ideal tissue adhesives are expected to have high biocompatibility, and be able to accelerate wound closing and healing, and monitor wound healing process. However, few adhesives fit all of the above descriptions. It has been demonstrated that inorganic nanoparticles can directly glue biological tissue based on nano-bridging effect. In this study, self-luminescence porous silicon (LPSi) particles were prepared with degradable and biocompatible properties. In addition, the self-luminescence property of LPSi particles was discovered by In Vivo Imaging System (IVIS) for the first time, which can avoid the limitations of photoluminescence imaging. Due to the oxidation and degradation reaction, LPSi particles not only can be degraded completely in several days, but also showed satisfactory biocompatibility. And their degradation product could promote tube formation of HUVECs. Moreover, owing to the high specific surface area and the outer oxide layer of LPSi particles, LPSi tissue adhesive exhibited strong adhesive strength to pig livers. Furthermore, this adhesive closed wound rapidly, promoted angiogenesis and epidermal regeneration, and facilitated wound healing in a mouse skin incision model. Importantly, the wound healing ratio can be monitored by measuring the self-luminescence intensity of LPSi particles in the wound site. This study reveals that LPSi particles could be employed as a safe and smart wound management tissue adhesive for wound closure, as well as accelerating and monitoring wound healing.
组织粘合剂因其在临床手术中有效密封伤口或切口的效果而受到广泛关注，特别是在微创手术中。为了满足安全和智能伤口管理的要求，理想的组织粘合剂需要具有良好的生物相容性，能够加速伤口闭合和愈合，并能监测伤口愈合过程。然而，很少有粘合剂能满足所有上述描述。研究表明，无机纳米粒子可以基于纳米桥接效应直接粘合生物组织。在本研究中，制备了具有可降解和生物相容性的自发光多孔硅（LPSi）颗粒。此外，首次通过体内成像系统（IVIS）发现了 LPSi 颗粒的自发光特性，这可以避免光致发光成像的局限性。由于氧化和降解反应，LPSi 颗粒不仅可以在几天内完全降解，而且表现出令人满意的生物相容性。其降解产物可以促进 HUVECs 的管形成。此外，由于 LPSi 颗粒的高比表面积和外氧化物层，LPSi 组织粘合剂对猪肝脏表现出很强的粘接力。此外，这种粘合剂可以快速闭合伤口，促进血管生成和表皮再生，促进小鼠皮肤切口模型中的伤口愈合。重要的是，可以通过测量伤口部位 LPSi 颗粒的自发光强度来监测伤口愈合的比率。这项研究表明，LPSi 颗粒可用作安全智能的伤口管理组织粘合剂，用于伤口闭合以及加速和监测伤口愈合。
