Shashidhar G M, Kumar S Sravan, Giridhar P, Manohar B
Food Engineering Department, CSIR-Central Food Technological Research Institute, Mysore, India.
Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, India.
Int J Med Mushrooms. 2017;19(4):337-345. doi: 10.1615/IntJMedMushrooms.v19.i4.40.
We investigated the potential use of coconut water to supplement potato dextrose broth (PDB) in the production of Ophiocordyceps sinensis CS1197 by submerged cultivation. The basal PDB medium was modified by supplementation with tender coconut water (TCW) and mature coconut water (MCW) at 10% and 5% (v/v), respectively; these mixtures were cultured at 28°C for 14 days, with a pH of 7 and an inoculum volume of 10%. The addition of optimized levels of TCW and MCW improved the biomass yield by 2.2- and 2.5-fold, respectively, and adenosine, cordycepin, and polysaccharide content by 58% and 69%, 50% and 55%, and 19% and 27%, respectively. Antioxidant and cholesterol esterase (CE) inhibitory activities of the aqueous extract from O. sinensis CS1197 mycelia supplemented with TCW and MCW were high compared with those of the control, indicating that coconut water has a positive correlation with the enhanced antioxidant and CE inhibitory activities. These antioxidant and CE inhibitory responses were dependent on concentration, and the larger amounts of bioactives in O. sinensis CS1197 are beneficial in pharmaceutical formulations.
我们研究了在深层培养中华被毛孢CS1197的过程中，使用椰子水替代马铃薯葡萄糖肉汤（PDB）的可能性。基础PDB培养基分别添加10%（v/v）的嫩椰子水（TCW）和5%（v/v）的成熟椰子水（MCW）进行改良；这些混合物在28°C下培养14天，pH值为7，接种量为10%。添加优化水平的TCW和MCW分别使生物量产量提高了2.2倍和2.5倍，腺苷、虫草素和多糖含量分别提高了58%和69%、50%和55%、19%和27%。与对照相比，添加TCW和MCW的中华被毛孢CS1197菌丝体水提取物的抗氧化和胆固醇酯酶（CE）抑制活性较高，表明椰子水与增强的抗氧化和CE抑制活性呈正相关。这些抗氧化和CE抑制反应取决于浓度，中华被毛孢CS1197中大量的生物活性物质在药物制剂中是有益的。
