Jiang Lan, Li Yi, Wang Wen-Jing, Imoulan Abdessamad, Yao Yi-Jian
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
Int J Med Mushrooms. 2017;19(1):35-44. doi: 10.1615/IntJMedMushrooms.v19.i1.40.
Paecilomyces hepialid, a fungus originating in the Tibetan Plateau, has been used as a substitute for Ophiocordyceps sinensis because the primary chemical compounds and pharmacological effects of P. hepialid are similar to those of O. sinensis. P. hepialid has been developed into a dietary supplement and pharmaceutical products. The antioxidant activity of extracts using 2 solvents (water and ethanol) from mycelia obtained from 2 cultivation modes (solid-state and submerged cultivation) were evaluated in this study. Four strains of P. hepialid obtained from Qinghai, Sichuan, and Yunnan Provinces were included; the total phenolic content and in vitro antioxidant activity of mycelial extracts were compared. The total phenolic content and antioxidant activity of strains were found to be affected by the cultivation mode and extraction solvent. The ethanol extracts of solid-state cultivation of strain 2138, obtained from Sichuan Province, exhibited the highest antioxidant activity. The results showed that different strains might require different cultivation modes and extraction solvents for better antioxidant activity. However, solid-state cultivation and ethanol extraction are generally recommended based on the analyses conducted. Strain 2138 may be a good candidate for the purpose of producing functional foods. The results suggest that strain selection is important when P. hepialid is used to manufacture pharmaceutical products.
蝙蝠蛾拟青霉，一种源自青藏高原的真菌，已被用作冬虫夏草的替代品，因为蝙蝠蛾拟青霉的主要化学成分和药理作用与冬虫夏草相似。蝙蝠蛾拟青霉已被开发成一种膳食补充剂和药品。本研究评估了从两种培养模式（固态培养和深层培养）获得的菌丝体，使用两种溶剂（水和乙醇）提取的提取物的抗氧化活性。研究包括从青海、四川和云南省获得的四株蝙蝠蛾拟青霉；比较了菌丝体提取物的总酚含量和体外抗氧化活性。发现菌株的总酚含量和抗氧化活性受培养模式和提取溶剂的影响。从四川省获得的2138菌株固态培养的乙醇提取物表现出最高的抗氧化活性。结果表明，不同菌株可能需要不同的培养模式和提取溶剂以获得更好的抗氧化活性。然而，根据所进行的分析，一般建议采用固态培养和乙醇提取。2138菌株可能是生产功能性食品的良好候选菌株。结果表明，在使用蝙蝠蛾拟青霉生产药品时，菌株选择很重要。
