Silva Maralina Torres da, Oliveira Cavalcante Pedro Hercílio de, Camargo Ana Carolina Alves de, Chagas Moutinho Vanessa Aparecida das, Santos Everton Gustavo Nunes Dos, Santos Cláudia Portes
Instituto Federal do Acre - IFAC, Rio Branco, AC, Brazil; Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fiocruz, Brazil.
Instituto Federal do Acre - IFAC, Xapuri, AC, Brazil; Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fiocruz, Brazil.
Vet Parasitol. 2017 Aug 15;242:14-21. doi: 10.1016/j.vetpar.2017.05.011. Epub 2017 May 21.
Arapaima gigas, a fish with a high market value, has been farmed in different localities within Brazil. Among its parasites, adults of Goezia spinulosa are reported to cause ulcers in the stomach and to result in the death of farmed fingerlings. Due to the veterinary importance of this nematode in cultured arapaimas, an integrative taxonomic study is proposed, combining morphological, ultrastructural and genetic profiles of this parasite. The fish were obtained from semi-intensive fish farming in Acre State, Brazil. The fish measured 7-42cm in total length and the intensity of infection was 1-60 parasites per fish. The site of infection was mainly the stomach. Morphological and ultrastructural analyses of G. spinulosa showed the importance of its spiny body in firmly attaching the worm to the gastric mucosa, resulting in lesions, ulcers and deep gastric perforations of the stomach wall. New sequences for partial 18S rDNA, ITS1, 5.8S and ITS2 rDNA, partial 28S rDNA, cox1 mtDNA and for cox2 mtDNA are presented. Phylogenetic reconstructions based on the partial 18S and 28S rDNA shows species of Goezia occur in a clade well separated from other genera in both analyses. Both the partial 18S and 28S rDNA genes represented good genetic markers for distinguishing genera of the Raphidascarididae, with exception of Hysterothylacium. This integrated taxonomic study produced a robust profile for G. spinulosa that will aid the diagnosis of both adults and larval stages from arapaimas and possible intermediate hosts.
巨骨舌鱼是一种具有高市场价值的鱼类，已在巴西的不同地区进行养殖。在其寄生虫中，据报道，棘口线虫的成虫会导致胃部溃疡，并导致养殖的幼鱼死亡。由于这种线虫对养殖的巨骨舌鱼具有兽医方面的重要性，因此提出了一项综合分类学研究，结合该寄生虫的形态学、超微结构和基因特征。这些鱼取自巴西阿克里州的半集约化养鱼场。鱼的全长为7 - 42厘米，感染强度为每条鱼1 - 60条寄生虫。感染部位主要是胃部。对棘口线虫的形态学和超微结构分析表明，其带刺的身体对于将蠕虫牢固地附着在胃黏膜上很重要，从而导致胃壁出现病变、溃疡和深部胃穿孔。本文给出了部分18S rDNA、ITS1、5.8S和ITS2 rDNA、部分28S rDNA、细胞色素氧化酶亚基1线粒体DNA（cox1 mtDNA）和细胞色素氧化酶亚基2线粒体DNA（cox2 mtDNA）的新序列。基于部分18S和28S rDNA的系统发育重建表明，在这两种分析中，棘口线虫属的物种都出现在一个与其他属明显分开的进化枝中。部分18S和28S rDNA基因都是区分蛔科属的良好遗传标记，但对异尖线虫属除外。这项综合分类学研究为棘口线虫生成了一个可靠的特征描述，这将有助于诊断巨骨舌鱼及其可能的中间宿主的成虫和幼虫阶段。
