Department of Pharmacy, Università 'G. d'Annunzio', Via dei Vestini, I-66100 Chieti, Italy.
Biomed Mater. 2017 Sep 13;12(5):055005. doi: 10.1088/1748-605X/aa7907.
Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.
商业胶原蛋白膜被用于口腔外科手术中，作为引导骨再生中骨沉积的支架。在这里，我们通过简单的非共价功能化方法将其与氧化石墨烯（GO）进行了富集，利用 GO 含氧碳官能团通过氢键与胶原蛋白相互作用的能力。在本文中，已经根据稳定性、纳米粗糙度、生物相容性和对人原代牙龈成纤维细胞炎症反应的诱导对 GO 涂层膜进行了表征。结果表明，所得到的涂层膜不会在本体溶液中漏出 GO，并且会改变膜的一些特性，如膜的刚性和膜与原子力显微镜（AFM）探针之间的粘附性。此外，如 AFM 分析所示，GO 的存在增加了粗糙度和暴露于细胞的总表面积。所得到的材料是生物相容的，并且不会在测试细胞中引起炎症。
