Radunovic Milena, De Colli Marianna, De Marco Patrizia, Di Nisio Chiara, Fontana Antonella, Piattelli Adriano, Cataldi Amelia, Zara Susi
Department of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.
Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara, Chieti, 66100, Italy.
J Biomed Mater Res A. 2017 Aug;105(8):2312-2320. doi: 10.1002/jbm.a.36085. Epub 2017 May 30.
Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 μg mL GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNFα and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNFα and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2312-2320, 2017.
胶原膜在口腔外科中用于治疗骨缺损，作为一种屏障，防止软组织侵入正在生长的骨组织。为了提高生物相容性，胶原膜被涂覆了氧化石墨烯（GO），一种石墨烯衍生物。本研究的目的是研究GO涂覆的胶原膜对人牙髓干细胞（DPSCs）的生物相容性，重点关注生物材料的细胞毒性、促进DPSCs分化过程的能力以及控制炎症事件诱导的能力。将DPSCs培养在未涂覆的膜以及2 μg/mL和10 μg/mL的GO涂覆膜上，培养长达28天。进行了alamar蓝和LDH细胞毒性测定、PGE2 ELISA测定、RUNX2、BMP2、SP7、TNFα和COX2基因表达的实时RT-PCR。在第14天和第28天，GO涂覆膜上的细胞增殖更高。LDH测定表明没有细胞毒性。涂覆膜上BMP2和RUNX2的表达更高，早期是BMP2，后期实验时间是RUNX2和SP7。在第14天和第28天，GO涂覆膜上的PGE2水平较低，当应用GO时，TNFα和COX2的表达均显著降低。GO涂覆膜对DPSCs无毒，可诱导DPSCs更快地分化为成牙本质细胞/成骨细胞，可能是传统膜的良好替代品，从而确保更有效的骨形成并改善临床性能。© 2017威利期刊公司。《生物医学材料研究杂志》A部分：105A：2312 - 2320，2017年。
